Trichostatin A down-regulate DNA methyltransferase 1 in Jurkat T cells

被引：50

作者：

Januchowski, Radoslaw ^{[1
]}

Dabrowski, Mikolaj ^{[1
]}

Ofori, Harold ^{[1
]}

Jagodzinski, Pawel P. ^{[1
]}

机构：

[1] Karol Marcinkowski Univ Med Sci, Dept Biochem & Mol Biol, PL-60781 Poznan, Poland

来源：

CANCER LETTERS | 2007年 / 246卷 / 1-2期

关键词：

trichostatin A; DNA methyltransferase 1; histone deacetylase; Jurkat T cells;

D O I：

10.1016/j.canlet.2006.03.010

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Histone deacetylase inhibitor Trichostatin A (TSA), alone, is able to activate the transcription of DNA methylation-mediated silenced genes in human cancer cells. Increase in expression and half-life of the DNA methyltransferase DNMT1 has been found in carcinomas of the colon, lung, liver, prostate, and breast cancer. This overexpression of DNMT1 is responsible for hypermethylation of regulatory sequences of many genes involved in tumorigenesis. Using quantitative real-time PCR and Western blot analysis, we found that TSA down-regulate DNMT1 mRNA and protein expression in Jurkat T leukemia cells clone E6-1. We also observed that TSA decreased DNMT1 mRNA stability and reduced this transcript half-life from approximately 7 to 2 h. We also found that protein biosynthesis is needed for posuranscriptional regulation of DNMT1 mRNA, which suggests the involvement of an RNase and/or mRNA stabilization protein entity in DNMT1 transcript stabilization. Our findings suggest that TSA not only alters histone acetylation, but also may affect DNA methylation. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：313 / 317

页数：5

共 20 条

[1] Increased protein stability causes DNA methyltransferase 1 dysregulation in breast cancer [J].