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Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay

被引：38
作者：
Hage, Chadi A.
Davis, Thomas E.
Egan, Lindsey
Parker, Michelle
Fuller, Deanna
LeMonte, Ann M.
Durkin, Michelle
Connelly, Patricia
Wheat, L. Joseph
Blue-Hnidy, Deborah
Knox, Kenneth S.
机构：
[1] Indiana Univ, Dept Med, Indianapolis, IN 46202 USA
[2] Indiana Univ, Dept Pathol & Lab Med, Indianapolis, IN 46202 USA
[3] MiraVista Diagnost & MiraBella Technol, Indianapolis, IN 46241 USA
来源：
RESPIRATORY MEDICINE | 2007年 / 101卷 / 01期
关键词：
histoplasmosis; blastomycosis; bronchoalveolar lavage; histoplasma antigen; second-generation assay;
D O I：
10.1016/j.rmed.2006.04.017
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Antigen detection is a useful adjunct for the diagnosis of histoptasmosis. The purpose of this study was to evaluate antigen detection in bronchoatveotar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoptasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoptasmosis and blastomycosis. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码：43 / 47
页数：5
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