Organization of a partial S10 operon and its transcriptional analysis in Flavobacterium hibernum strain W22

被引：4

作者：

Chen, Shicheng

Bagdasarian, Michael

Kaufman, Michael G.

Walker, Edward D. ^{[1
]}

机构：

[1] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

[2] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 267卷 / 01期

关键词：

ribosomal protein; Flavobacterium hibernum; transcription;

D O I：

10.1111/j.1574-6968.2006.00543.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cluster of six genes from Flavobacterium hibernum strain W22, fus-rpsJ-rplC-rplD-rplW-rplB, was cloned and sequenced. A short fragment upstream of rplC, but not of rpsJ, showed strong promoter activity in flavobacteria. TATCTTTG and TTG motifs that are conserved in Flavobacterium promoters were found immediately upstream of the transcription start point of rplC, at the -7 and -33 positions, respectively. RT-PCR analysis of the transcripts revealed that rpsJ and rplC are expressed as separate transcriptional units, whereas rplC and rplD-rplW-rplB are cotranscribed as a single mRNA, in contrast to the situation in the Gammaproteobacteria, which have a single transcriptional unit of 11 ribosomal S10 genes.

引用

页码：38 / 45

页数：8

共 18 条

[1] Cloning and characterization of the Flavobacterium johnsoniae (Cytophaga johnsonae) gliding motility gene, gldA [J].