Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs

被引:170
作者
Labarque, GG [1 ]
Nauwynck, HJ [1 ]
Van Reeth, K [1 ]
Pensaert, MB [1 ]
机构
[1] State Univ Ghent, Fac Vet Med, Virol Lab, B-9820 Merelbeke, Belgium
关键词
D O I
10.1099/0022-1317-81-5-1327
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Twenty-two 4- to 5-week-old gnotobiotic pigs were intranasally inoculated with 10(6.0) TCID50 of porcine reproductive and respiratory syndrome virus (PRRSV) (Lelystad) and euthanized at different time intervals post-inoculation (p,i,), Bronchoalveolar lavage (BAL) cell populations were characterized, together with the pattern of virus replication and appearance of antibodies in the lungs. Total BAL cell numbers increased from 140 x 10(6) at 5 days p,i, to 948 x 10(6) at 25 days p,i, and remained at high levels until the end of the experiment. The number of monocytes/macrophages, as identified by monoclonal antibodies 74-22-15 and 41D3, increased two- to fivefold between 9 and 52 days p,i, with a maximum at 25 days p,i, Flow cytometry showed that the population of differentiated macrophages was reduced between 9 and 20 days p,i, and that between the same time interval, both 74-22-15-positive and 41D3-negative cells, presumably monocytes, and 74-22-15-positive and 41D3-double negative cells, presumably non-phagocytes, entered the alveolar spaces. Virus replication was highest at 7 to 9 days p,i,, decreased slowly thereafter and was detected until 40 days p,i, Anti-PRRSV antibodies were detected starting at 9 days p,i, but neutralizing antibodies were only demonstrated in one pig euthanized at 35 days and another at 52 days p.i, The decrease of virus replication in the lungs from 9 days p,i, can be attributed to (i) shortage of susceptible differentiated macrophages, (ii) lack of susceptibility of the newly infiltrated monocytes and (iii) appearance of anti-PRRSV antibodies in the lungs. Neutralizing antibodies may contribute to the clearance of PRRSV from the lungs.
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页码:1327 / 1334
页数:8
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