Reliable amplification method for bacterial RNA

被引：12

作者：

Rachman, Helmy ^{[1
]}

Lee, Jong Seok ^{[1
]}

Angermann, Joerg ^{[1
]}

Kowall, Jane ^{[1
]}

Kaufmann, Stefan H. E. ^{[1
]}

机构：

[1] Max Planck Inst Infect Biol, Dept Immunol, D-10117 Berlin, Germany

来源：

JOURNAL OF BIOTECHNOLOGY | 2006年 / 126卷 / 01期

关键词：

RNA amplification; microarray; mycobacteria;

D O I：

10.1016/j.jbiotec.2006.02.020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA microarray technology has been increasingly applied for studies of clinical samples. Frequently, RNA probes from clinical samples are available in limited amounts. We describe a reliable amplification method for bacterial RNA. We verified this method on mycobacterial RNA applying mycobacterial genome-directed primers (mtGDPs). Glass slide-based oligoarrays were employed to assess the quality of the amplification method. We observed a relatively small bias in amplified RNA pool when compared to the unamplified one. Up to 1000-fold linear RNA amplification in a single amplification round was obtained. To our knowledge, this study describes the first amplification method for mycobacterial RNA. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：61 / 68

页数：8

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