Novel swine influenza virus subtype H3N1 in Italy

被引：20

作者：

Moreno, Ana ^{[1
]}

Barbieri, Ilaria ^{[1
]}

Sozzi, Enrica ^{[1
]}

Luppi, Andrea ^{[1
]}

Lelli, Davide ^{[1
]}

Lombardi, Guerino ^{[1
]}

Zanoni, Maria Grazia ^{[1
]}

Cordioli, Paolo ^{[1
]}

机构：

[1] IZSLER, Dept Anim Hlth, Virol Lab, I-25124 Brescia, Italy

来源：

VETERINARY MICROBIOLOGY | 2009年 / 138卷 / 3-4期

关键词：

Swine; Novel subtype H3N1; Genetic characterization; Italy; A VIRUS; GENETIC-CHARACTERIZATION; H1N2; VIRUS; PIGS; HEMAGGLUTININ; NUCLEOPROTEIN; AMPLIFICATION; REASSORTMENT; REPLICATION; EMERGENCE;

D O I：

10.1016/j.vetmic.2009.04.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To date, three subtypes of swine influenza viruses, H1N1, H1N2, and H3N2 have been isolated in Italy. In 2006, a novel swine influenza virus subtype (H3N1) was isolated from coughing pigs. RT-PCR performed on lung tissues, experimental infection in pigs with the novel isolate, and cloning the virus by plaque assay confirmed this unique H and N combination. The novel isolate was also antigenically and genetically characterized. Genetic and phylogenetic analysis showed that the complete HA gene of the H3N1 strain has the highest nucleotide identity to three Italian H3N2 strains, one isolated in 2001 and two in 2004, whereas the full length NA sequence is closely related to three H1N1 subtype viruses isolated in Italy in 2004. The remaining genes are also closely related to respective genes found in H1N1 and H3N2 SIVs currently circulating in Italy. This suggests that the novel SIV could be a reassortant between the H3N2 and H1N1 SIVs circulating in Italy. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：361 / 367

页数：7

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