Plasmodium vivax synonymous substitution frequencies, evolution and population structure deduced from diversity in AMA 1 and MSP 1 genes

被引:93
作者
Figtree, M
Pasay, CJ
Slade, R
Cheng, Q
Cloonan, N
Walker, J
Saul, A [1 ]
机构
[1] Queensland Inst Med Res, Australian Ctr Int & Trop Med, Brisbane, Qld 4006, Australia
[2] Univ Queensland, Brisbane, Qld, Australia
[3] Univ Sydney, Sydney, NSW 2006, Australia
[4] Res Inst Trop Med, Muntinlupa, Metro Manila, Philippines
[5] Westmead Hosp, Sydney, NSW, Australia
基金
美国国家卫生研究院; 英国医学研究理事会;
关键词
malaria; Plasmodium vivax; apical membrane antigen 1; merozoite surface protein 1; population structure; synonymous;
D O I
10.1016/S0166-6851(00)00204-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Polymorphic regions of the genes encoding Plasmodium vivax apical membrane antigen 1 (PvAMA1) and P. vivax merozoite surface protein 1 (PvMSP1) were sequenced to examine population diversity both within and between geographical areas. Sequences were obtained for 219 isolates for PvAMA1 and for 175 isolates for PvMSP1 from Africa, China, India, Indonesia, Philippines, Papua New Guinea, Solomon Islands and Thailand. Over half of the isolates were obtained from different regions within the Philippines, and this was used to look at the diversity within a country. Sixty nine haplotypes and 22 polymorphic sites in a 414-bp region of PvAMA1 and 41 haplotypes and 34 polymorphic sites in a 249-bp fragment of PvMSP1 were detected. For both PvAMA1 and PvMSP1, four previously unreported polymorphic nucleotide positions were identified. Population analysis indicated that there were significant differences in allele frequencies between different regions but these differences were small compared to the diversity within populations (Fixation index, F-ST, of 0.126 and 0.078 for PvAMA1 and PvMSP1, respectively). PvAMA1 and PvMSP1 had similar nonsynonymous substitution frequencies but surprisingly, the synonymous substitution fre-quency for PvMSP1 was eight times the frequency for PvAMA1 suggesting that synonymous substitutions in at least PvAMA1 are not neutral. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:53 / 66
页数:14
相关论文
共 50 条
  • [31] MUTATIONS WITH MULTIPLE INDEPENDENT ORIGINS IN SURFACE-ANTIGENS MARK THE TARGETS OF BIOLOGICAL SELECTIVE PRESSURE
    MCCUTCHAN, TF
    WATERS, AP
    [J]. IMMUNOLOGY LETTERS, 1990, 25 (1-3) : 23 - 26
  • [32] VIVAX MALARIA RESISTANT TO TREATMENT AND PROPHYLAXIS WITH CHLOROQUINE
    MURPHY, GS
    BASRI, H
    PURNOMO
    ANDERSEN, EM
    BANGS, MJ
    MOUNT, DL
    GORDEN, J
    LAL, AA
    PURWOKUSUMO, AR
    HARJOSUWARNO, S
    SORENSEN, K
    HOFFMAN, SL
    [J]. LANCET, 1993, 341 (8837) : 96 - 100
  • [33] NEI M, 1986, MOL BIOL EVOL, V3, P418
  • [34] DIMORPHISM OF THE C-TERMINUS OF THE PLASMODIUM-VIVAX MEROZOITE SURFACE PROTEIN-1
    PASAY, MC
    CHENG, Q
    RZEPCZYK, C
    SAUL, A
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1995, 70 (1-2) : 217 - 219
  • [35] APICAL MEMBRANE ANTIGEN OF PLASMODIUM-FRAGILE
    PETERSON, MG
    NGUYENDINH, P
    MARSHALL, VM
    ELLIOTT, JF
    COLLINS, WE
    ANDERS, RF
    KEMP, DJ
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 39 (02) : 279 - 284
  • [36] PLASMODIUM-VIVAX - RECOMBINATION BETWEEN POTENTIAL ALLELIC TYPES OF THE MEROZOITE SURFACE PROTEIN MSP1 IN PARASITES ISOLATED FROM PATIENTS
    PREMAWANSA, S
    SNEWIN, VA
    KHOURI, E
    MENDIS, KN
    DAVID, PH
    [J]. EXPERIMENTAL PARASITOLOGY, 1993, 76 (02) : 192 - 199
  • [37] Interallelic recombination in the merozoite surface protein 1 (MSP-1) gene of Plasmodium vivax from Thai isolates
    Putaporntip, C
    Jongwutiwes, S
    Tanabe, K
    Thaithong, S
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1997, 84 (01) : 49 - 56
  • [38] POLYMORPHISM IN THE CIRCUMSPOROZOITE PROTEIN OF THE HUMAN MALARIA PARASITE PLASMODIUM-VIVAX
    QARI, SH
    GOLDMAN, IF
    POVOA, MM
    DISANTI, S
    ALPERS, MP
    LAL, AA
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 55 (1-2) : 105 - 113
  • [39] QARI SH, 1991, J BIOL CHEM, V266, P16297
  • [40] Rain A. Noor, 1993, Southeast Asian Journal of Tropical Medicine and Public Health, V24, P388