Antisense down regulation of NtBI-1 in tobacco BY-2 cells induces accelerated cell death upon carbon starvation

被引：40

作者：

Bolduc, N ^{[1
]}

Brisson, LF ^{[1
]}

机构：

[1] Univ Laval, Dept Biochem & Microbiol, Quebec City, PQ G1K 7P4, Canada

来源：

FEBS LETTERS | 2002年 / 532卷 / 1-2期

关键词：

Bax inhibitor-1; programmed cell death; DNA fragmentation; plant; autophagy;

D O I：

10.1016/S0014-5793(02)03650-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bax inhibitor-1 (BI-1) protein is proposed to be a conserved programmed cell death suppressor. In this report, we investigate the anti-apoptotic function of plant BI-1 by antisense (AS) down regulation of NtBI-1 in Nicotiana tabacum cv. BY-2 cells. We observed that AS cell lines were more susceptible to autophagy, internucleosomal DNA fragmentation and death than control cells when subjected to sucrose starvation and hypo-osmotic shock, in agreement with a role of BI-1 as a death inhibitor. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：111 / 114

页数：4

共 14 条

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