Micellar electrokinetic capillary chromatography of benzodiazepine antiepileptics and their desmethyl metabolites in blood

被引：22

作者：

Imazawa, M

Hatanaka, Y

机构：

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1997年 / 15卷 / 9-10期

关键词：

micellar electrokinetic chromatography; benzodiazepines; antiepileptics; human serum; electrophoresis; sample stacking; sodium dodecylsulphate; serum;

D O I：

10.1016/S0731-7085(97)00037-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A fast and reliable method for the MEKC separation and determination of benzodiazepine antipileptics and their active metabolites in serum has been developed, using a separation buffer composed of berate (pH 9.5), SDS (18 mM), and acetonitrile (14%) as an organic modifier. The method is sensitive enough to be used clinically with a precision of less than 3% for the analysis of nitrazepam. clonazepam, clobazam, diazepam and their desmethyl metabolites in serum. (C) 1997 Elsevier Science B.V.

引用

页码：1503 / 1508

页数：6