p.Asn176Lys and p.Met137Thr dimorphisms of the PRNP gene significantly decrease the susceptibility to classical scrapie in ARQ/ARQ sheep

被引：10

作者：

Maestrale, C. ^{[1
]}

Carta, A. ^{[2
]}

Attene, S. ^{[1
]}

Galistu, A. ^{[1
]}

Santucciu, C. ^{[1
]}

Cancedda, M. G. ^{[1
]}

Saba, M. ^{[1
]}

Sechi, S. ^{[2
]}

Patta, C. ^{[1
]}

Bandino, E. ^{[1
]}

Ligios, C. ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Sardegna, I-07100 Sassari, Italy

[2] AGRIS Sardinia, Dept Res Anim Prod, I-07040 Olmedo, Italy

来源：

ANIMAL GENETICS | 2009年 / 40卷 / 06期

关键词：

PRNP gene; scrapie; sheep; BOVINE SPONGIFORM ENCEPHALOPATHY; NATURAL SCRAPIE; BRITISH SHEEP; PRP; RESISTANCE; VARIANT; BSE;

D O I：

10.1111/j.1365-2052.2009.01943.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

P>In this study, we investigated the susceptibility to scrapie of Sarda breed sheep carrying the genotype ARQ/ARQ with additional polymorphisms at the PRNP gene. To do this, we examined 256 scrapie-affected sheep and 320 flock-mate negative controls from 24 flocks. Logistic regression analysis demonstrated that sheep carrying the ARQ/ARQ genotype with additional dimorphisms had lower risk of becoming scrapie affected when compared with those with ARQ/ARQ(wildtype) genotype. ARQ/ARQ genotypes that were detected with heterozygous or homozygous p.Asn176Lys and p.Met137Thr dimorphisms were associated with the lowest susceptibility to the disease. A significant lower risk was also associated with the p.Arg154His dimorphism, while p.Leu141Phe had a protective effect that was not statistically significant.

引用

页码：982 / 985

页数：4

共 16 条

[1] SCRAPIE AND CELLULAR PRP ISOFORMS ARE ENCODED BY THE SAME CHROMOSOMAL GENE [J].

BASLER, K ;

OESCH, B ;

SCOTT, M ;

WESTAWAY, D ;

WALCHLI, M ;

GROTH, DF ;

MCKINLEY, MP ;

PRUSINER, SB ;

WEISSMANN, C .

CELL, 1986, 46 (03) :417-428

[2] The genetics of scrapie in sheep and goats [J].

Baylis, M ;

Goldmann, W .

CURRENT MOLECULAR MEDICINE, 2004, 4 (04) :385-396

[3] PrP genotype contributes to determining survival times of sheep with natural scrapie [J].

Bossers, A ;

Schreuder, BEC ;

Muileman, IH ;

Belt, PBGM ;

Smits, MA .

JOURNAL OF GENERAL VIROLOGY, 1996, 77 :2669-2673

[4]

Dawson M, 1998, VET REC, V142, P623

[5] Frequencies of PrP gene haplotypes in British sheep flocks and the implications for breeding programmes [J].

Goldmann, W ;

Baylis, M ;

Chihota, C ;

Stevenson, E ;

Hunter, N .

JOURNAL OF APPLIED MICROBIOLOGY, 2005, 98 (06) :1294-1302

[6] Ovine prion protein variant A136 R154L168Q171 increases resistance to experimental challenge with bovine spongiform encephalopathy agent [J].

Goldmann, Wilfred ;

Houston, Fiona ;

Stewart, Paula ;

Perucchini, Matteo ;

Foster, James ;

Hunter, Nora .

JOURNAL OF GENERAL VIROLOGY, 2006, 87 :3741-3745

[7] THE ASSOCIATION OF A CODON-136 PRP GENE VARIANT WITH THE OCCURRENCE OF NATURAL SCRAPIE [J].

HUNTER, N ;

GOLDMANN, W ;

SMITH, G ;

HOPE, J .

ARCHIVES OF VIROLOGY, 1994, 137 (1-2) :171-177

[8] NATURAL SCRAPIE IN BRITISH SHEEP - BREEDS, AGES AND PRP GENE POLYMORPHISMS [J].

HUNTER, N ;

FOSTER, JD ;

HOPE, J .

VETERINARY RECORD, 1992, 130 (18) :389-392

[9] PrP genetics in sheep and the implications for scrapie and BSE [J].

Hunter, N .

TRENDS IN MICROBIOLOGY, 1997, 5 (08) :331-334

[10] Scrapie Resistance in ARQ Sheep [J].

Laegreid, W. W. ;

Clawson, M. L. ;

Heaton, M. P. ;

Green, B. T. ;

O'Rourke, K. I. ;

Knowles, D. P. .

JOURNAL OF VIROLOGY, 2008, 82 (20) :10318-10320

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