Protein and lipid composition of bovine milk-fat-globule membrane

被引：220

作者：

Fong, Bertram Y. ^{[1
]}

Norris, Carmen S. ^{[1
]}

MacGibbon, Alastair K. H. ^{[1
]}

机构：

[1] Fonterra Res Ctr, Palmerston North, New Zealand

来源：

INTERNATIONAL DAIRY JOURNAL | 2007年 / 17卷 / 04期

关键词：

milk-fat-globule membrane; butyrophilin; phospholipids; lipids; mass spectrometry;

D O I：

10.1016/j.idairyj.2006.05.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Using mass spectrometry and other analytical methods, the proteins and lipids isolated from bovine milk-fat-globule membrane (MFGM) were characterised. The major MFGM protein composition consisted of xanthine oxidase, butyrophilin, adipophilin and periodic acid schiff 6/7. The minor proteins were polymeric immunoglobulin receptor protein, apolipoprotein E, apolipoprotein A1, 71 kDa heat-shock cognate protein, clusterin, lactoperoxidase, immunoglobulin heavy chain and peptidylprolyl isomerase A, actin, fatty acid-binding protein, cluster of differentiation 26 and mucin. The MFGM lipid component consisted predominantly of triglycerides (56%) and phospholipids (40.6%). The major fatty acids associated with the glycerol phospholipids were C16:0, C18:0, C18:1 and C18:2. Sphingomyelin had a high proportion of C20:0, C23:0, C24:1 and C24:0 fatty acids linked to the sphingoloid base. However, the sphingoloid base itself consisted predominantly of C16:1, C17:1 and C18:1 fatty acids. Small amounts of both lactosyl- and glucosyl-cerebrosides were found in the bovine MFGM sample and trace levels of lyso-phosphatidyl ethanolamine and lyso-phosphatidyl choline were detected. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：275 / 288

页数：14

共 65 条

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