Identification of a role for actin in translational fidelity in yeast

被引:25
作者
Kandl, KA
Munshi, R
Ortiz, PA
Andersen, GR
Kinzy, TG
Adams, AEM [1 ]
机构
[1] Univ Arizona, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA
[2] St Olaf Coll, Dept Biol, Northfield, MN 55057 USA
[3] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA
[4] Canc Inst New Jersey, Piscataway, NJ 08854 USA
[5] Aarhus Univ, Inst Mol & Struct Biol, DK-8000 Aarhus, Denmark
[6] Univ Sussex, Dept Biochem, Brighton BN1 9QG, E Sussex, England
关键词
actin cytoskeleton; translation; nonsense suppression;
D O I
10.1007/s00438-002-0726-x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Numerous studies have suggested a role for actin in translation, but the molecular details of this role are unknown. To elucidate the function(s) of actin in translation, we have studied 25 isogenic, conditional yeast actin mutants. Strikingly, analysis of these mutants indicates that none of those tested have conditional growth defects caused by reduced rates of protein synthesis; and analysis of latrunculin A-treated wild-type cells indicates that even complete disruption of the actin cytoskeleton has no significant effect on the rate of translation. However, analysis of the effect of the 25 actin mutations on fidelity and sensitivity to translation inhibitors identified two mutations (act1-2 and act1-122) that cause a significant reduction in the fidelity of translation, as assayed by nonsense suppression, and several mutants that are sensitive to paromomycin, which affects translational fidelity. Translation elongation factor 1A (eEF1A) also has a role in fidelity, and in the presence of excess eEF1A four of the mutants (act1-2, act1-20, act1-120, and act1-125) are even more sensitive to paromomycin, while one mutant (act1-122) becomes less sensitive. Together, these findings suggest that actin may not be important for the rate of translation, but may have a critical role in ensuring translational fidelity.
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页码:10 / 18
页数:9
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