Method for inactivating and fixing unstained smear preparations of Mycobacterium tuberculosis for improved laboratory safety

被引：25

作者：

Chedore, P

Th'ng, C

Nolan, DH

Churchwell, GM

Sieffert, DE

Hale, YM

Jamieson, F

机构：

[1] Minist Hlth & Long Term Care, Labs Branch, Toronto, ON M9P 3T1, Canada

[2] Bur Labs, Florida Dept Hlth, Jacksonville, FL USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 11期

关键词：

D O I：

10.1128/JCM.40.11.4077-4080.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The inactivation of smears that contain Mycobacterium tuberculosis for microscopy before removal of the material from a biosafety cabinet is an important safety factor in preventing the potential transmission of tuberculosis to laboratory workers. The fixing and inactivating properties of heat flaming, 70% ethanol, and 1, 3, and 5% phenol in ethanol for smears containing M. tuberculosis were investigated. Heat flaming failed to inactivate the smear material, whereas 5% phenol in ethanol successfully fixed and inactivated all smears containing M. tuberculosis both from concentrated sputum samples and from culture material.

引用

页码：4077 / 4080

页数：4

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