Modulation by lipopolysaccharide of inflammatory cytokine production by two T cell lines

this study, the modulation of inflammatory cytokine production by lipopolysaccharide (LPS) in two T cell lines, RL-male-1 and L5178Y-ML was examined, Both cell lines produced interleukin 1 (IL-1) activity in response to LPS, which was largely independent of the presence of serum, The IL-1 activity induced was neutralized by an anti-IL-1 beta antibody, but not by an anti-IL-1 alpha antibody, Induction of IL-1 alpha and beta was also confirmed by polymerase chain reaction of reverse-transcribed mRNA (RT-PCR), although in RL-male-1 cells, IL-1 alpha mRNA was constitutively expressed and somewhat enhanced by LPS, RT-PCR analysis revealed that these cell lines also upregulated tumour necrosis factor alpha (TNF-alpha) and IL-1 receptor antagonist mRNAs in response to LPS, although RL-male-1 cells expressed TNF-alpha mRNA constitutively, Flow cytometric analysis showed that, although these cells expressed Thy-1 antigen, they hardly expressed CD14 and gamma delta T cell receptor, In conclusion, LPS modulated inflammatory cytokine production in these T cell lines. (C) 1997 Academic Press.