Association between Lifestyle Factors and CpG Island Methylation in a Cancer-Free Population

被引：54

作者：

Brait, Mariana ^{[1
]}

Ford, Jean G. ^{[2
,3
]}

Papaiahgari, Srinivas ^{[2
]}

Garza, Mary A. ^{[6
]}

Lee, Jin I. ^{[3
]}

Loyo, Myriam ^{[1
]}

Maldonado, Leonel ^{[1
]}

Begum, Shahnaz ^{[5
]}

McCaffrey, Lee ^{[3
]}

Howerton, Mollie ^{[2
,3
]}

Sidransky, David ^{[1
]}

Emerson, Mark R. ^{[4
]}

Ahmed, Saifuddin ^{[4
]}

Williams, Carla D. ^{[7
]}

Hoque, Mohammad Obaidul ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Otolaryngol Head & Neck Surg, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21231 USA

[3] Johns Hopkins Univ, Johns Hopkins Bloomberg Sch Publ Hlth, Dept Epidemiol, Baltimore, MD 21231 USA

[4] Johns Hopkins Univ, Johns Hopkins Bloomberg Sch Publ Hlth, Dept Populat Family & Reprod Hlth, Baltimore, MD 21231 USA

[5] Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA

[6] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Behav & Community Hlth Sci, Pittsburgh, PA USA

[7] Howard Univ, Sch Med, Dept Med, Washington, DC 20059 USA

来源：

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION | 2009年 / 18卷 / 11期

关键词：

ABERRANT PROMOTER METHYLATION; CELL LUNG-CANCER; MULTIPLE GENES; PROSTATE-CANCER; URINE SEDIMENT; BLADDER-CANCER; NEVER-SMOKERS; PLASMA DNA; HYPERMETHYLATION; SMOKING;

D O I：

10.1158/1055-9965.EPI-08-1245

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Many risk factors have been associated with cancer, such as age, family history, race, smoking, high-fat diet, and poor nutrition. It is important to reveal the molecular changes related to risk factors that could facilitate early detection, prevention, and overall control of cancer. Methods: We selected six cancer-specific methylated genes that have previously been reported in primary tumors and have also been detected in different bodily fluids of cancer patients. Here, we used quantitative fluorogenic real-time methylation-specific PCR in plasma DNA samples for the detection of methylation changes from an asymptomatic population who do not have any known cancer. Results: The promoter methylation frequencies of the studied genes were as follows: APC (7%), CCND2 (22%), GSTP1 (2%), MGMT (9%), RAR beta 2 (29%), and P16 (3%). Promoter methylation of at least one of the genes analyzed was observed in similar to 46% (72 of 157) of the samples by binary dichotomization. Promoter hypermethylation of at least two genes was detected in 17% (26 of 157) of the samples. RAR beta 2 methylation was observed in 45% of subjects who had a high-fat diet in contrast with those who had a low-fat diet (23%; P = 0.007). Discussion: Our findings may help to elucidate early methylation changes that may lead to cancer development. These methylation changes could be due to exposure to risk factors and may be useful for cancer prevention measures such as changes in lifestyle. Longitudinal follow-up of a high-risk population is needed to understand the association of methylation of candidate genes in cancer development. (Cancer Epidemiol Biomarkers Prev 2009;18(11):2984-91)

引用

页码：2984 / 2991

页数：8

共 47 条

[1]

Ahijevych Karen L, 2002, Nicotine Tob Res, V4, P423, DOI 10.1080/1462220021000018452

[2] Predicting gene promoter methylation in non-small-cell lung cancer by evaluating sputum and serum [J].

Belinsky, S. A. ;

Grimes, M. J. ;

Casas, E. ;

Stidley, C. A. ;

Franklin, W. A. ;

Bocklage, T. J. ;

Johnson, D. H. ;

Schiller, J. H. .

BRITISH JOURNAL OF CANCER, 2007, 96 (08) :1278-1283

[3] Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort [J].

Belinsky, SA ;

Liechty, KC ;

Gentry, FD ;

Wolf, HJ ;

Rogers, J ;

Vu, K ;

Haney, J ;

Kenned, TC ;

Hirsch, FR ;

Miller, Y ;

Franklin, WA ;

Herman, JG ;

Baylin, SB ;

Bunn, PA ;

Byers, T .

CANCER RESEARCH, 2006, 66 (06) :3338-3344

[4] Aberrant promoter methylation of multiple genes during pathogenesis of bladder cancer [J].

Brait, Mariana ;

Begum, Shahnaz ;

Carvalho, Andre L. ;

Dasgupta, Santanu ;

Vettore, Andre L. ;

Czemiak, Bogdan ;

Caballero, Otavia L. ;

Westra, William H. ;

Sidransky, David ;

Hoque, Mohammad Obaidul .

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2008, 17 (10) :2786-2794

[5] Concordant CpG island methylation in hyperplastic polyposis [J].

Chan, AOO ;

Issa, JPJ ;

Morris, JS ;

Hamilton, SR ;

Rashid, A .

AMERICAN JOURNAL OF PATHOLOGY, 2002, 160 (02) :529-536

[6] Dietary fat intake and risk of prostate cancer in the European Prospective Investigation into Cancer and Nutrition [J].

Crowe, Francesca L. ;

Key, Timothy J. ;

Appleby, Paul N. ;

Travis, Ruth C. ;

Overvad, Kim ;

Jakobsen, Marianne U. ;

Johnsen, Nina F. ;

Tjonneland, Anne ;

Linseisen, Jakob ;

Rohrmann, Sabine ;

Boeing, Heiner ;

Pischon, Tobias ;

Trichopoulou, Antonia ;

Lagiou, Pagona ;

Trichopoulos, Dimitrios ;

Sacerdote, Carlotta ;

Palli, Domenico ;

Tumino, Rosario ;

Krogh, Vitorrio ;

Bueno-de-Mesquita, H. Bas ;

Kiemeney, Lambertus A. ;

Chirlaque, Maria-Dolores ;

Ardanaz, Eva ;

Sanchez, Maria-Jose ;

Larranaga, Nerea ;

Gonzalez, Carlos A. ;

Quiros, Jose R. ;

Manjer, Jonas ;

Wirfalt, Elisabet ;

Stattin, Par ;

Hallmans, Goran ;

Khaw, Kay-Tee ;

Bingham, Sheila ;

Ferrari, Pietro ;

Slimani, Nadia ;

Jenab, Mazda ;

Riboli, Elio .

AMERICAN JOURNAL OF CLINICAL NUTRITION, 2008, 87 (05) :1405-1413

[7]

de Caceres Inmaculada Ibanez, 2004, Cancer Research, V64, P6476, DOI 10.1158/0008-5472.CAN-04-1529

[8] DNA hypomethylation, cancer, the immunodeficiency, centromeric region instability, facial anomalies syndrome and chromosomal rearrangements [J].

Ehrlich, M .

JOURNAL OF NUTRITION, 2002, 132 (08) :2424S-2429S

[9]

Esteller M, 2000, CANCER RES, V60, P4366

[10] Environmental and nutritional effects on the epigenetic regulation of genes [J].

Feil, Robert .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2006, 600 (1-2) :46-57

← 1 2 3 4 5 →