Genome sequence of Shigella flexneri 2a:: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157

被引:350
作者
Jin, Q [1 ]
Yuan, ZH
Xu, JG
Wang, Y
Shen, Y
Lu, WC
Wang, JH
Liu, H
Yang, J
Yang, F
Zhang, XB
Zhang, JY
Yang, GW
Wu, HT
Qu, D
Dong, J
Sun, LL
Xue, Y
Zhao, AL
Gao, YS
Zhu, JP
Kan, B
Ding, KY
Chen, SX
Cheng, HS
Yao, ZJ
He, BK
Chen, RS
Ma, DL
Qiang, BQ
Wen, YM
Hou, YD
Yu, J
机构
[1] State Key Lab Mol Virol & Genet Engn, Beijing 100052, Peoples R China
[2] Fudan Univ, Mol Virol Lab, Shanghai 200032, Peoples R China
[3] Chinese Acad Prevent Med, Inst Epidemiol & Microbiol, Beijing 102206, Peoples R China
[4] Peking Univ, Hlth Sci Ctr, Beijing 100083, Peoples R China
[5] Natl Ctr Human Genome Res, Beijing 100176, Peoples R China
[6] Chinese Acad Sci, Inst Biophys, Lab Bioinformat, Beijing 100101, Peoples R China
[7] Chinese Minist Publ Hlth, Microbial Genome Ctr, Beijing 100052, Peoples R China
[8] Beijing Microbial Genome Res Ctr, Beijing 100052, Peoples R China
[9] HuaBei Pharmaceut Co Ltd, Shijiazhuang 0500, Peoples R China
[10] Univ London Imperial Coll Sci & Technol, Fac Med, Dept Paediat, Mol Infect Dis Grp, London W2 1PG, England
关键词
D O I
10.1093/nar/gkf566
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have sequenced the genome of Shigella flexneri serotype 2a, the most prevalent species and serotype that causes bacillary dysentery or shigellosis in man. The whole genome is composed of a 4 607 203 bp chromosome and a 221 618 bp virulence plasmid, designated pCP301. While the plasmid shows minor divergence from that sequenced in serotype 5a, striking characteristics of the chromosome have been revealed. The S.flexneri chromosome has, astonishingly, 314 IS elements, more than 7-fold over those possessed by its close relatives, the non-pathogenic K12 strain and enterohemorrhagic O157:H7 strain of Escherichia coli. There are 13 translocations and inversions compared with the E.coli sequences, all involve a segment larger than 5 kb, and most are associated with deletions or acquired DNA sequences, of which several are likely to be bacteriophage-transmitted pathogenicity islands. Furthermore, S.flexneri, resembling another human-restricted enteric pathogen, Salmonella typhi, also has hundreds of pseudogenes compared with the E.coli strains. All of these could be subjected to investigations towards novel preventative and treatment strategies against shigellosis.
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收藏
页码:4432 / 4441
页数:10
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