Photoswitching of the fluorescent protein asFP595:: Mechanism, proton pathways, and absorption spectra

被引：89

作者：

Schaefer, Lars V.

Groenhof, Gerrit

Klingen, Astrid R.

Ullmann, G. Matthias

Boggio-Pasqua, Martial

Robb, Michael A.

Grubmueller, Helmut

机构：

[1] Max Planck Inst Biophys Chem, Abt Theoret & Comp Gestutzte Biophys, D-37077 Gottingen, Germany

[2] Univ Bayreuth, Abt Strukturbiol Bioinformat, D-95447 Bayreuth, Germany

[3] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

absorption spectroscopy; isomerization; molecular dynamics; proteins; proton transport;

D O I：

10.1002/anie.200602315

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Molecular light-switch: Off-on switching of the fluorescence of the protein asFP595 involves a trans-cis isomerization. Mixed quantum/classical simulations elucidate the spectroscopic properties of asFP595 and give detailed insights into the photoswitching mechanism. The conformational trans-cis switching triggers a proton-transfer cascade between the chromophore and adjacent amino acids. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：530 / 536

页数：7

相关论文

共 53 条

[1] Proton pathways in green fluorescence protein [J].

Agmon, N .

BIOPHYSICAL JOURNAL, 2005, 88 (04) :2452-2461

[2] Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting [J].

Ando, R ;

Mizuno, H ;

Miyawaki, A .

SCIENCE, 2004, 306 (5700) :1370-1373

[3] Structure and mechanism of the reversible photoswitch of a fluorescent protein [J].

Andresen, M ;

Wahl, MC ;

Stiel, AC ;

Gräter, F ;

Schäfer, LV ;

Trowitzsch, S ;

Weber, G ;

Eggeling, C ;

Grubmüller, H ;

Hell, SW ;

Jakobs, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13070-13074

[4] INTERMEDIATE NEGLECT OF DIFFERENTIAL OVERLAP THEORY FOR TRANSITION-METAL COMPLEXES - FE, CO AND CU CHLORIDES [J].

BACON, AD ;

ZERNER, MC .

THEORETICA CHIMICA ACTA, 1979, 53 (01) :21-54

[5] PKAS OF IONIZABLE GROUPS IN PROTEINS - ATOMIC DETAIL FROM A CONTINUUM ELECTROSTATIC MODEL [J].

BASHFORD, D ;

KARPLUS, M .

BIOCHEMISTRY, 1990, 29 (44) :10219-10225

[6] DENSITY-FUNCTIONAL EXCHANGE-ENERGY APPROXIMATION WITH CORRECT ASYMPTOTIC-BEHAVIOR [J].

BECKE, AD .

PHYSICAL REVIEW A, 1988, 38 (06) :3098-3100

[7] Probing the ground state structure of the green fluorescent protein chromophore using Raman spectroscopy [J].

Bell, AF ;

He, X ;

Wachter, RM ;

Tonge, PJ .

BIOCHEMISTRY, 2000, 39 (15) :4423-4431

[8] Crystal structure of halophilic dodecin:: A novel, dodecameric flavin binding protein from Halobacterium salinarum [J].

Bieger, B ;

Essen, LO ;

Oesterhelt, D .

STRUCTURE, 2003, 11 (04) :375-385

[9]

Casida ME, 1995, RECENT ADV DENSITY F

[10] Kindling fluorescent proteins for precise in vivo photolabeling [J].

Chudakov, DM ;

Belousov, VV ;

Zaraisky, AG ;

Novoselov, VV ;

Staroverov, DB ;

Zorov, DB ;

Lukyanov, S ;

Lukyanov, KA .

NATURE BIOTECHNOLOGY, 2003, 21 (02) :191-194

← 1 2 3 4 5 6 →