Photoswitching of the fluorescent protein asFP595:: Mechanism, proton pathways, and absorption spectra

被引：89

作者：

Schaefer, Lars V.

Groenhof, Gerrit

Klingen, Astrid R.

Ullmann, G. Matthias

Boggio-Pasqua, Martial

Robb, Michael A.

Grubmueller, Helmut

机构：

[1] Max Planck Inst Biophys Chem, Abt Theoret & Comp Gestutzte Biophys, D-37077 Gottingen, Germany

[2] Univ Bayreuth, Abt Strukturbiol Bioinformat, D-95447 Bayreuth, Germany

[3] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

absorption spectroscopy; isomerization; molecular dynamics; proteins; proton transport;

D O I：

10.1002/anie.200602315

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Molecular light-switch: Off-on switching of the fluorescence of the protein asFP595 involves a trans-cis isomerization. Mixed quantum/classical simulations elucidate the spectroscopic properties of asFP595 and give detailed insights into the photoswitching mechanism. The conformational trans-cis switching triggers a proton-transfer cascade between the chromophore and adjacent amino acids. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：530 / 536

页数：7

相关论文

共 53 条

[11] Chromophore environment provides clue to "kindling fluorescent protein" riddle [J].

Chudakov, DM ;

Feofanov, AV ;

Mudriku, NN ;

Lukyanov, S ;

Lukyanov, KA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (09) :7215-7219

[12]

Crano JC, 1999, T APPL CHEM, V1, P1

[13] Lack of negative charge in the E46Q mutant of photoactive yellow protein prevents partial unfolding of the blue-shifted intermediate [J].

Derix, NM ;

Wechselberger, RW ;

van der Horst, MA ;

Hellingwerf, KJ ;

Boelens, R ;

Kaptein, R ;

van Nuland, NAJ .

BIOCHEMISTRY, 2003, 42 (49) :14501-14506

[14]

Frisch M. J., 2004, GAUSSIAN 03 REVISION

[15] QM/MM study of energy storage and molecular rearrangements due to the primary event in vision [J].

Gascon, JA ;

Batista, VS .

BIOPHYSICAL JOURNAL, 2004, 87 (05) :2931-2941

[16] trans and cis Chrornophore structures in the kindling fluorescent protein asFP595 [J].

Grigorenko, Bella ;

Savitsky, Alexander ;

Topol, Igor ;

Burt, Stanley ;

Nemukhin, Alexander .

CHEMICAL PHYSICS LETTERS, 2006, 424 (1-3) :184-188

[17] Photoactivation of the photoactive yellow protein: Why photon absorption triggers a trans-to-cis lsomerization of the chromophore in the protein [J].

Groenhof, G ;

Bouxin-Cademartory, M ;

Hess, B ;

De Visser, SP ;

Berendsen, HJC ;

Olivucci, M ;

Mark, AE ;

Robb, MA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (13) :4228-4233

[18] Signal transduction in the photoactive yellow protein. I. Photon absorption and the isomerization of the chromophore [J].

Groenhof, G ;

Lensink, MF ;

Berendsen, HJC ;

Snijders, JG ;

Mark, AE .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2002, 48 (02) :202-211

[19] Reversible single-molecule photoswitching in the GFP-like fluorescent protein Dronpa [J].

Habuchi, S ;

Ando, R ;

Dedecker, P ;

Verheijen, W ;

Mizuno, H ;

Miyawaki, A ;

Hofkens, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (27) :9511-9516

[20] Evidence for the isomerization and decarboxylation in the photoconversion of the red fluorescent protein DsRed [J].

Habuchi, S ;

Cotlet, M ;

Gensch, T ;

Bednarz, T ;

Haber-Pohlmeier, S ;

Rozenski, J ;

Dirix, G ;

Michiels, J ;

Vanderleyden, J ;

Heberle, J ;

De Schryver, FC ;

Hofkens, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (25) :8977-8984

← 1 2 3 4 5 6 →