Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods

被引：34

作者：

Blais, BW ^{[1
]}

Gaudreault, M ^{[1
]}

Phillippe, LM ^{[1
]}

机构：

[1] Canadian Food Inspect Agcy, Lab Serv Div, CEF, Ottawa, ON K1A 0C6, Canada

来源：

FOOD CONTROL | 2003年 / 14卷 / 01期

关键词：

multiplex; enzyme immunoassay; allergens; peanut; hazelnut; Brazil nut;

D O I：

10.1016/S0956-7135(02)00053-1

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An enzyme immunoassay (EIA) system was developed for the simultaneous detection of multiple allergens in foods. This assay utilized a reverse dot blot EIA format in which egg yolk antibodies (IgY) specific for different allergens were immobilized in discrete spots on a strip of polyester cloth, which was then contacted with the test sample. The binding of antigens to a specific spot on the strip was revealed by sequential reactions with a pool of biotinylated allergen-specific antibodies, streptavidin-peroxidase conjugate and a chromogenic substrate. As an example of the applicability of this multiplex EIA system, we demonstrate the detection of three important food allergens: peanut, hazelnut and Brazil nut. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：43 / 47

页数：5

共 13 条

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