Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods

被引:34
作者
Blais, BW [1 ]
Gaudreault, M [1 ]
Phillippe, LM [1 ]
机构
[1] Canadian Food Inspect Agcy, Lab Serv Div, CEF, Ottawa, ON K1A 0C6, Canada
关键词
multiplex; enzyme immunoassay; allergens; peanut; hazelnut; Brazil nut;
D O I
10.1016/S0956-7135(02)00053-1
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
An enzyme immunoassay (EIA) system was developed for the simultaneous detection of multiple allergens in foods. This assay utilized a reverse dot blot EIA format in which egg yolk antibodies (IgY) specific for different allergens were immobilized in discrete spots on a strip of polyester cloth, which was then contacted with the test sample. The binding of antigens to a specific spot on the strip was revealed by sequential reactions with a pool of biotinylated allergen-specific antibodies, streptavidin-peroxidase conjugate and a chromogenic substrate. As an example of the applicability of this multiplex EIA system, we demonstrate the detection of three important food allergens: peanut, hazelnut and Brazil nut. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:43 / 47
页数:5
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