Experimental and Computational Characterization of Biological Liquid Crystals: A Review of Single-Molecule Bioassays

被引：10

作者：

Eom, Kilho ^{[1
]}

Yang, Jaemoon ^{[2
]}

Park, Jinsung ^{[1
]}

Yoon, Gwonchan ^{[1
]}

Sohn, Young Soo ^{[3
]}

Park, Shinsuk ^{[1
]}

Yoon, Dae Sung ^{[4
]}

Na, Sungsoo ^{[1
]}

Kwon, Taeyun ^{[5
]}

机构：

[1] Korea Univ, Dept Mech Engn, Seoul 136701, South Korea

[2] Yonsei Univ, Dept Radiol, Coll Med, Seoul 120752, South Korea

[3] Catholic Univ Daegu, Dept Biomed Engn, Kyeongbuk 712702, South Korea

[4] Yonsei Univ, Dept Biomed Engn, Kangwon Do 220740, South Korea

[5] Korea Univ, Res Inst Engn & Technol, Seoul 136701, South Korea

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2009年 / 10卷 / 09期

关键词：

single-molecule experiments; atomic force microscope (AFM); coarse-grained molecular dynamics simulation; coarse-grained model; in vitro molecular recognitions; ATOMIC-FORCE MICROSCOPY; AMPLITUDE CONFORMATIONAL FLUCTUATIONS; COLLECTIVE VARIABLE DESCRIPTION; DYNAMICS SIMULATIONS; UNFOLDING PATHWAYS; ENERGY LANDSCAPE; SECONDARY STRUCTURES; GLOBULAR-PROTEINS; ADHESION BONDS; DNA-MOLECULES;

D O I：

10.3390/ijms10094009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative understanding of the mechanical behavior of biological liquid crystals such as proteins is essential for gaining insight into their biological functions, since some proteins perform notable mechanical functions. Recently, single-molecule experiments have allowed not only the quantitative characterization of the mechanical behavior of proteins such as protein unfolding mechanics, but also the exploration of the free energy landscape for protein folding. In this work, we have reviewed the current state-of-art in single-molecule bioassays that enable quantitative studies on protein unfolding mechanics and/or various molecular interactions. Specifically, single-molecule pulling experiments based on atomic force microscopy (AFM) have been overviewed. In addition, the computational simulations on single-molecule pulling experiments have been reviewed. We have also reviewed the AFM cantilever-based bioassay that provides insight into various molecular interactions. Our review highlights the AFM-based single-molecule bioassay for quantitative characterization of biological liquid crystals such as proteins.

引用

页码：4009 / 4032

页数：24

共 114 条

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