Development and evaluation of specific 16S rDNA primers for marine Cytophaga-Flavobacteria cluster

被引：12

作者：

Chen, Xihan ^{[1
]}

Zeng, Yonghui ^{[1
]}

Jiao, Nianzhi ^{[1
]}

机构：

[1] Xiamen Univ, Natl Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 04期

关键词：

16S rDNA; clone library; Cytophaga-Flavobacteria; diversity; marine environments; primer design;

D O I：

10.1111/j.1471-8286.2006.01514.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Through multiple alignment analysis of 16S rDNA sequences from all known genera of Cytophaga-Flavobacteria (CF) cluster, a new primer pair specifically targeting this cluster was developed, greatly facilitating their diversity and function's exploration in marine ecosystems. Compared with previously reported primers, the new primer pair could theoretically retrieve broader CF diversity without decreasing specificity. The effectiveness for field samples was further evaluated by testing the community DNA samples from various marine environments using the optimal polymerase chain reaction (PCR) conditions established in this work. The results showed its robustness and high specificity for amplifying CF cluster's 16S rDNA fragments from complex marine environments.

引用

页码：1278 / 1281

页数：4

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