High-performance liquid chromatographic analysis of amino acid- and peptide-derived chloramines

被引:16
作者
Furness-Green, SM [1 ]
Inskeep, TR [1 ]
Starke, JJ [1 ]
Ping, L [1 ]
Greenleaf-Schumann, HR [1 ]
Goyne, TE [1 ]
机构
[1] Valparaiso Univ, Dept Chem, Valparaiso, IN 46383 USA
关键词
D O I
10.1093/chromsci/36.5.227
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An isocratic reversed-phase high-performance liquid chromatographic method is reported for the analysis of amino acid- and peptide-derived chloramines (these are important intermediates in two very different processes: destruction of microbes by the neutrophil and disinfection of water with chlorine). Specifically, results are reported for the chloramines derived from the following amino acids and dipeptides: taurine, Ala, Gly, Ser, Thr, Phe, Val, AlaGLy, GlyGly, PheGly, SerGly, and ValGly. Analyses are performed on a 250 × 4.6-mm C18 column using a buffered water-acetonitrile mixture as the mobile phase. For samples containing hydrophilic chloramines, an ion-pairing agent is added to the mobile phase. Two detection methods are used: direct ultraviolet (UV) detection of the chloramine at 254 nm and indirect UV detection of I3- at 350 nm following postcolumn reaction with iodide. Unexpectedly, the decomposition of amino acid-derived chloramines is found to greatly accelerate during chromatographic elution on a reversed-phase column.
引用
收藏
页码:227 / 236
页数:10
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