The CatWalk method: A detailed analysis of behavioral changes after acute inflammatory pain in the rat

被引:111
作者
Gabriel, A. F.
Marcus, M. A. E.
Honig, W. M. M.
Walenkamp, G. H. I. M.
Joosten, E. A. J.
机构
[1] Acad Hosp Maastricht, Dept Anesthesiol, NL-6200 MD Maastricht, Netherlands
[2] Acad Hosp Maastricht, Dept Surg & Orthoped, NL-6200 MD Maastricht, Netherlands
[3] Univ Maastricht, EURON, European Grad Sch Neurosci, NL-6200 MD Maastricht, Netherlands
关键词
CatWalk; carrageenan; acute pain; gait analysis; mechanical allodynia;
D O I
10.1016/j.jneumeth.2007.02.003
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Experimental pain research is often complicated by the absence of an objective and detailed method to analyze behavioral changes. In the present study, acute pain was induced into the right knee of the rat (n = 15) through the injection of 2 mg carrageenan (CAR) in saline. A control group received vehicle injection into the knee (n = 15). With the use of an automated quantitative gait analysis system, the CatWalk, it was possible to quantitatively analyze behavioral changes at post-injection time 2.5, 4, 24 and 48 h. The CatWalk analysis of individual paw parameters like the intensity of the paw print or the time contact with the floor showed a significant effect after CAR injection into the knee. These CatWalk parameters were highly correlated with von Frey data and thus representative for the development of mechanical allodynia. Furthermore, detailed CatWalk analysis of the gait (i.e. coordinated interaction between left and right hindlimb) showed very fine, accurate and significant coordination changes in the experimental rats from 4 h post-injection. In conclusion, the CatWalk method allows an objective and detailed detection of both pain-induced gait adaptations as well as the development of mechanical allodynia in an acute inflammatory pain model. (c) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:9 / 16
页数:8
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