Simultaneous determination of tryptophan and kynurenine in serum by HPLC with UV and fluorescence detection

被引：59

作者：

Vignau, J

Jacquemont, MC

Lefort, A

Imbenotte, M

Lhermitte, M

机构：

[1] CHRU, CITD, Serv Addictol Clin Psychiat, Lille, France

[2] Fac Sci Pharmaceut & Biol, Toxicol Lab, F-59006 Lille, France

来源：

BIOMEDICAL CHROMATOGRAPHY | 2004年 / 18卷 / 10期

关键词：

mental depression; tryptophan; kynurenine production; blood serum; HPLC; indoleamine 2,3-dioxygenase (IDO);

D O I：

10.1002/bmc.445

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tryptophan metabolism is disturbed in mental depression, and the induction of indoleamine 2,3-dioxygenase increases kynurenine production. In order to determine this disturbance in patients with chronic hepatitis C and receiving interferon-based immunotherapy, a new and specific HPLC protocol was elaborated. For tryptophan, the assay was linear from 6.25 to 100 mumol L-1, and the limits of detection (LOD) and quantitation (LOQ) for the method were 0.7 and 8.0 mumol L-1. For kynurenine, the linearity of calibration was from 0.0625 to 6.25 mumol L-1, with LOD and LOQ of 2 and 3 nmol L-1. Reproducibility and repeatability were satisfactory. The method allowed study of human blood serum. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：872 / 874

页数：3

共 6 条

[1] Increased depressive ratings in patients with hepatitis C receiving interferon-α-based immunotherapy are related to interferon-α-induced changes in the serotonergic system [J].