Determination of tryptophan and its kynurenine pathway metabolites in human serum by high-performance liquid chromatography with simultaneous ultraviolet and fluorimetric detection

被引：159

作者：

Herve, C

Beyne, P

Jamault, H

Delacoux, E

机构：

[1] Service de Biochimie, Hôpital Beaujon 100, F-92110 Clichy, Bld du Général Leclerc

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1996年 / 675卷 / 01期

关键词：

tryptophan; kynurenine; 3-hydroxykynurenine; kynurenic acid; 3-hydroxyanthranilic acid;

D O I：

10.1016/0378-4347(95)00341-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isocratic reversed-phase high-performance liquid chromatographic method for the simultaneous determination of tryptophan and four metabolites of the kynurenine pathway (kynurenine, 3-hydroxykynurenine, kynurenic acid and 3-hydroxyanthranilic acid) in human serum is described. This new method, which uses both isocratic elution and two on-line connected programmable ultraviolet and spectrofluorimetric detectors, allows the determination of these metabolites, in the physiological ranges, with satisfying specificity and sensitivity within 30 min.

引用

页码：157 / 161

页数：5

共 19 条

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