Sample stacking revisited: A personal perspective

被引:123
作者
Chien, RL [1 ]
机构
[1] Caliper Technol Corp, Mountain View, CA 94043 USA
关键词
electrokinetics; electroosmosis; field-amplified sample stacking; microchips; microfluidic device; review; sample preconcentration; sample stacking;
D O I
10.1002/elps.200390057
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
One of the major challenges in capillary electrophoresis and other miniaturization separation techniques is to maintain high detection sensitivity in the increasingly smaller dimension. Numerous on-column sample preconcentrating procedures, based either on electrokinetic focusing or chromatographic effects, have been developed. This review will discuss some practical approaches to sample stacking from a personal perspective. Several recent developments in sample stacking on microfluidic devices are reviewed.
引用
收藏
页码:486 / 497
页数:12
相关论文
共 117 条
[81]   ELECTROOSMOSIS IN CAPILLARY ZONE ELECTROPHORESIS WITH NONUNIFORM ZETA-POTENTIAL [J].
POTOCEK, B ;
GAS, B ;
KENNDLER, E ;
STEDRY, M .
JOURNAL OF CHROMATOGRAPHY A, 1995, 709 (01) :51-62
[82]   Sweeping of neutral analytes in electrokinetic chromatography with high-salt-containing matrixes [J].
Quirino, JP ;
Terabe, S ;
Bocek, P .
ANALYTICAL CHEMISTRY, 2000, 72 (08) :1934-1940
[83]   Sample stacking of cationic and anionic analytes in capillary electrophoresis [J].
Quirino, JP ;
Terabe, S .
JOURNAL OF CHROMATOGRAPHY A, 2000, 902 (01) :119-135
[84]  
Quirino JP, 1999, HRC-J HIGH RES CHROM, V22, P367
[85]  
Quirino JP, 2000, ELECTROPHORESIS, V21, P355, DOI 10.1002/(SICI)1522-2683(20000101)21:2<355::AID-ELPS355>3.3.CO
[86]  
2-4
[87]  
Quirino JP, 1997, J CAPILLARY ELECTROP, V4, P233
[88]   Approaching a million-fold sensitivity increase in capillary electrophoresis with direct ultraviolet detection: Cation-selective exhaustive injection and sweeping [J].
Quirino, JP ;
Terabe, S .
ANALYTICAL CHEMISTRY, 2000, 72 (05) :1023-1030
[89]   Sweeping of analyte zones in electrokinetic chromatography [J].
Quirino, JP ;
Terabe, S .
ANALYTICAL CHEMISTRY, 1999, 71 (08) :1638-1644
[90]  
QUIRINO JP, 1998, CHROMATOGRAPHY, V19, P246