Effectiveness of cross-linked phyllosilicates for intercalative immobilization of soybean lipoxygenase

被引：14

作者：

Shen, SY ^{[1
]}

Hsu, AF ^{[1
]}

Foglia, TA ^{[1
]}

Tu, SI ^{[1
]}

机构：

[1] USDA, ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19118 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 69卷 / 02期

关键词：

lipoxygenase; enzyme immobilization; cross-linked phyllosilicates; intercalation;

D O I：

10.1007/BF02919390

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel procedure was developed to intercalate enzymes into dispersed phyllosilicates that were cross-linked with silicate polymers formed by the hydrolysis of tetramethyl orthosilicate (TMOS). Lipoxygenase (LOX) intercalated into cross-linked phyllosilicates exhibited high enzymatic activity. The enzyme-phyllosilicate composite prepared by this procedure had an improved pore network. Alkylamines were used to occupy the charge sites of the phyllosilicate, which increased the hydrophobicity of the phyllosilicate and reduced charge-charge interaction between LOX and the phyllosilicate. The amount of macropores and the enzymatic activity of the Lipoxygenase-phyllosilicate composites increased with an increase in the ratio of trimethylammonium (TMA)-phyllosilicate to cross-linking reagent TMOS. LOX intercalatively immobilized into phyllosilicates displayed good storage stability and reusability at ambient temperature.

引用

页码：79 / 90

页数：12

共 19 条

[1] ADSORPTION OF VARIOUS ENZYMES ONTO TALCS SCOPE AND LIMITATIONS OF THIS NEW IMMOBILIZATION SYSTEM [J].