The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes

被引：35

作者：

Ledda, S ^{[1
]}

Loi, P ^{[1
]}

Bogliolo, L ^{[1
]}

Moor, RM ^{[1
]}

Fulka, J ^{[1
]}

机构：

[1] UNIV SASSARI,INST ZOOTECHN,OLMEDO,ITALY

来源：

ZYGOTE | 1996年 / 4卷 / 01期

关键词：

activation; DNA synthesis; oocytes;

D O I：

10.1017/S0967199400002835

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethylaminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.

引用

收藏

页码：7 / 9

页数：3

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