Response of isoprene emission and carbon metabolism to drought in white poplar (Populus alba) saplings

被引:233
作者
Brilli, Federico
Barta, Csengele
Fortunati, Alessio
Lerdau, Manuel
Loreto, Francesco
Centritto, Mauro
机构
[1] CNR, Ist Inquinamento Atmosfer, I-00016 Monterotondo, Italy
[2] CNR, Ist Biol Agroambientale & Forestale, I-00016 Monterotondo, Italy
[3] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22904 USA
关键词
C-13; labeling; intercellular CO2 concentration; isoprene synthase activity and concentration; photosynthesis; stomatal conductance; transpirable soil water; volatile organic compounds; water stress;
D O I
10.1111/j.1469-8137.2007.02094.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The mechanism uncoupling isoprene emission and photosynthesis under drought was investigated in Populus alba saplings. Isoprene emission, incorporation of C-13 into the isoprene molecule, isoprene synthase (ISPS) activity, concentration and gene expression, and photosynthesis were measured as a function of the fraction of transpirable soil water (FTSW) and in plants recovering from drought. Photosynthesis sharply declined below FTSW30 (a FTSW of 30%) and its inhibition was not caused by metabolic factors. A decline in isoprene emission was only evident towards the FTSW endpoint. C-13 incorporation into isoprene was lower when photosynthesis was constrained by drought. ISPS activity was inhibited by mild drought, while ISPS gene expression and concentration declined in concert with isoprene emission at the FTSW endpoint. Following rewatering, isoprene emission was higher than, and photosynthesis was similar to, prestress values. ISPS activity and concentration, and C-13 incorporation into isoprene, also rapidly recovered to prestress values, while ISPS gene expression remained low in rewatered plants. Our experiment revealed a larger contribution of alternative carbon sources to isoprene emission only when photosynthesis was dramatically constrained by drought. Isoprene emission was likely controlled at the posttranscriptional level under severe drought.
引用
收藏
页码:244 / 254
页数:11
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