Submillimolar levels of calcium regulates DNA structure at the dinucleotide repeat (TG/AC)n

被引:50
作者
Dobi, A [1 ]
von Agoston, D [1 ]
机构
[1] NICHHD, Dev Neurobiol Lab, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1073/pnas.95.11.5981
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Submillimolar levels of calcium, similar to the physiological total (bound + free) intranuclear concentration (0.01-1 mM), induced a conformational change within d(TG/AC)(n), one of the frequent dinucleotide repeats of the mammalian genome. This change is calcium-specific, because no other tested cation induced it and it was detected as a concentration-dependent transition from B- to a non-B-DNA conformation expanding from 3' end toward the 5' of the repeat. Genomic footprinting of various rat brain regions revealed the existence of similar non-B-DNA conformation within a d(TG/AC)(28) repeat of the endogenous enkephalin gene only in enkephalin-expressing caudate nucleus and not in the nonexpressing thalamus. Binding assays demonstrated that DNA could bind calcium and can compete with calmodulin for calcium.
引用
收藏
页码:5981 / 5986
页数:6
相关论文
共 47 条