Expression, crystallization and activities of the two family 11 aldo-keto reductases from Bacillus subtilis

被引：7

作者：

Ehrensberger, A ^{[1
]}

Wilson, DK ^{[1
]}

机构：

[1] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2003年 / 59卷

关键词：

D O I：

10.1107/S0907444902072001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two members of the aldo-keto reductase family 11 from Bacillus subtilis have been crystallized and their oxidoreductase activity conrrmed. AKR11A is a protein induced by inositol and repressed by glucose. AKR11B is induced when the cell is stressed by heat, acid, ethanol, starvation or osmotic shock and is therefore classified as a general stress protein. The expected NADPH-dependent sugar reductase activities for both proteins have been conrrmed kinetically with several substrates. AKR11B exhibited typical aldo-keto reductase kinetics. However, only trace activity was found in AKR11A. To examine the effects of differences in sequence on the structures and functions of these enzymes, a crystallographic study has been initiated. AKR11A has been crystallized in its apo form and AKR11B crystals were obtained in complex with NADP(+).

引用

页码：375 / 377

页数：3

共 19 条

[11] Processing of X-ray diffraction data collected in oscillation mode [J].

Otwinowski, Z ;

Minor, W .

MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :307-326

[12] Identification and transcriptional analysis of new members of the σB regulon in Bacillus subtilis [J].

Petersohn, A ;

Antelmann, H ;

Gerth, U ;

Hecker, M .

MICROBIOLOGY-UK, 1999, 145 :869-880

[13]

RAMALEY R, 1979, J BIOL CHEM, V254, P7684

[14] NOVEL NADPH-BINDING DOMAIN REVEALED BY THE CRYSTAL-STRUCTURE OF ALDOSE REDUCTASE [J].

RONDEAU, JM ;

TETEFAVIER, F ;

PODJARNY, A ;

REYMANN, JM ;

BARTH, P ;

BIELLMANN, JF ;

MORAS, D .

NATURE, 1992, 355 (6359) :469-472

[15] AN UNLIKELY SUGAR SUBSTRATE SITE IN THE 1.65 ANGSTROM STRUCTURE OF THE HUMAN ALDOSE REDUCTASE HOLOENZYME IMPLICATED IN DIABETIC COMPLICATIONS [J].

WILSON, DK ;

BOHREN, KM ;

GABBAY, KH ;

QUIOCHO, FA .

SCIENCE, 1992, 257 (5066) :81-84

[16]

Ye QL, 2000, PROTEINS, V38, P41, DOI 10.1002/(SICI)1097-0134(20000101)38:1<41::AID-PROT5>3.0.CO

[17]

2-M

[18] Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis [J].

Yoshida, K ;

Kobayashi, K ;

Miwa, Y ;

Kang, CM ;

Matsunaga, M ;

Yamaguchi, H ;

Tojo, S ;

Yamamoto, M ;

Nishi, R ;

Ogasawara, N ;

Nakayama, T ;

Fujita, Y .

NUCLEIC ACIDS RESEARCH, 2001, 29 (03) :683-692

[19] Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis [J].

Yoshida, KI ;

Aoyama, D ;

Ishio, I ;

Shibayama, T ;

Fujita, Y .

JOURNAL OF BACTERIOLOGY, 1997, 179 (14) :4591-4598

← 1 2 →