A rapid-format antibody card test for diagnosis of onchocerciasis

被引:98
作者
Weil, GJ
Steel, C
Liftis, F
Li, BW
Mearns, G
Lobos, E
Nutman, TB
机构
[1] Barnes Jewish Hosp, Div Infect Dis, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, St Louis, MO USA
[3] NIH, Parasit Dis Lab, Bethesda, MD 20892 USA
[4] AMRAD ICT, Sydney, NSW, Australia
[5] UCL, London, England
来源
JOURNAL OF INFECTIOUS DISEASES | 2000年 / 182卷 / 06期
关键词
D O I
10.1086/317629
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Improved methods are needed for field diagnosis of onchocerciasis, to support efforts aimed at elimination of the disease. A rapid-format card test was evaluated that detects IgG4 antibodies to recombinant Onchocerca volvulus antigen Ov16 with serum samples from patients with onchocerciasis and with various types of control serum samples. The sensitivity of the test with serum samples from 106 microfilariae-positive subjects was 90.6%. The test was equally sensitive with serum samples obtained from patients in Africa and Latin America. Specificity was excellent; positive tests were observed for 2 of 38 serum samples from patients with other filarial infections and for 1 of 23 serum samples from patients with nonfilarial helminth infections. The 3 "falsepositive" serum samples were from West Africans who could have been coinfected with onchocerciasis. No positive tests were observed with nonendemic serum samples from normal adults, patients with autoimmune disorders, or patients with the hyper-IgE syndrome. This new test holds great promise as a simple tool for diagnosis of onchocerciasis.
引用
收藏
页码:1796 / 1799
页数:4
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