Single-lens theta microscopy - a new implementation of confocal theta microscopy

被引：15

作者：

Lindek, S ^{[1
]}

Stefany, T ^{[1
]}

Stelzer, EHK ^{[1
]}

机构：

[1] European Mol Biol Lab, Cell Biophys Programme, Light Microscopy Grp, D-69012 Heidelberg, Germany

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1997年 / 188卷

关键词：

4Pi microscopy; confocal; fluorescence; numerical aperture; resolution; theta microscopy; water-immersion objective lenses;

D O I：

10.1046/j.1365-2818.1997.2650822.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A new microscopical technique based on the principle of confocal theta microscopy (Stelzer, E.H.K., Lindek, S. & Pick, R. (1996) Konfokales Mikroskop. German Patent Office DE 43 26 473 (filed 6.8.1993, granted 6.12.1996)) is described. It uses a single objective lens in combination with a mirror unit to achieve the theta configuration that leads to axial and volume resolution improvements. In this paper we present technical details of possible microscopical set-ups, and we discuss different versions of mirror units.

引用

页码：280 / 284

页数：5

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