Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci

被引：374

作者：

Tulin, A ^{[1
]}

Spradling, A ^{[1
]}

机构：

[1] Carnegie Inst Sci, Dept Embryol, Howard Hughes Med Res Labs, Baltimore, MD 21210 USA

来源：

SCIENCE | 2003年 / 299卷 / 5606期

关键词：

D O I：

10.1126/science.1078764

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Steroid response and stress-activated genes such as hsp70 undergo puffing in Drosophila larval salivary glands, a local loosening of polytene chromatin structure associated with gene induction. We find that puffs acquire elevated levels of adenosine diphosphate ( ADP)-ribose modified proteins and that poly( ADP) ribose polymerase ( PARP) is required to produce normal-sized puffs and normal amounts of Hsp70 after heat exposure. We propose that chromosomal PARP molecules become activated by developmental or environmental cues and strip nearby chromatin proteins off DNA to generate a puff. Such local loosening may facilitate transcription and may transiently make protein complexes more accessible to modi cation, promoting chromatin remodeling during development.

引用

页码：560 / 562

页数：4

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