A lipid's eye view of membrane protein crystallization in mesophases

被引：156

作者：

Caffrey, M ^{[1
]}

机构：

[1] Ohio State Univ, Dept Chem, Columbus, OH 43210 USA

[2] Ohio State Univ, Ohio State Biophys Program, Columbus, OH 43210 USA

[3] Ohio State Univ, Ohio State Biochem Program, Columbus, OH 43210 USA

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2000年 / 10卷 / 04期

关键词：

D O I：

10.1016/S0959-440X(00)00119-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipidic mesophases have been used to produce diffraction-quality crystals of several membrane proteins. The mechanism by which the method works is a mystery. The thrust is to continue to use it whilst deciphering the underlying mechanism and solving the second 'phase problem' in membrane protein crystallography. The method, which probably shares similarities with crystal growth in microgravity, is examined here from a lipid and a phase science perspective.

引用

页码：486 / 497

页数：12

共 51 条

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