Design and optimization of a near-field scanning optical microscope for imaging biological samples in liquid

被引：31

作者：

Gheber, LA ^{[1
]}

Hwang, J ^{[1
]}

Edidin, M ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

来源：

APPLIED OPTICS | 1998年 / 37卷 / 16期

关键词：

D O I：

10.1364/AO.37.003574

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a near-field scanning optical microscope capable of imaging biological samples in liquid. The microscope uses a straight optical fiber near-field probe and optical sheer-force feedback for tip sample distance regulation. Physical aspects of the design are discussed, and phenomena related to operation in liquid are revealed. Careful calibration of the instrument in air and in liquid is shown, and for the first time to our knowledge, near-field fluorescence images of a biological cell in liquid are presented. (C) 1998 Optical Society of America.

引用

页码：3574 / 3581

页数：8

共 31 条

[1] COMBINED SHEAR FORCE AND NEAR-FIELD SCANNING OPTICAL MICROSCOPY [J].

BETZIG, E ;

FINN, PL ;

WEINER, JS .

APPLIED PHYSICS LETTERS, 1992, 60 (20) :2484-2486

[2] ATOMIC FORCE MICROSCOPE [J].

BINNIG, G ;

QUATE, CF ;

GERBER, C .

PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933

[3] SCANNING TUNNELING MICROSCOPY [J].

BINNIG, G ;

ROHRER, H .

SURFACE SCIENCE, 1983, 126 (1-3) :236-244

[4]

*EG G OPT CAN, SINGL PHOT COUNT MOD

[5]

*EG G PRINC APPL R, LOCK IN AMPL MOD 521

[6]

*ESCO PROD INC, 0 310 IN 0 079 CM DI

[7]

Gregor MJ, 1996, APPL PHYS LETT, V68, P307, DOI 10.1063/1.116068

[8] A NONOPTICAL TIP-SAMPLE DISTANCE CONTROL METHOD FOR NEAR-FIELD SCANNING OPTICAL MICROSCOPY USING IMPEDANCE CHANGES IN AN ELECTROMECHANICAL SYSTEM [J].

HSU, JWP ;

LEE, M ;

DEAVER, BS .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1995, 66 (05) :3177-3181

[9] PIEZOELECTRIC TIP-SAMPLE DISTANCE CONTROL FOR NEAR-FIELD OPTICAL MICROSCOPES [J].

KARRAI, K ;

GROBER, RD .

APPLIED PHYSICS LETTERS, 1995, 66 (14) :1842-1844

[10] Scanning near-field optical microscopy in reflection mode imaging in liquid [J].

Keller, TH ;

Rayment, T ;

Klenerman, D ;

Stephenson, RJ .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1997, 68 (03) :1448-1454

← 1 2 3 4 →