Turning protein crystallisation from an art into a science

被引:169
作者
Chayen, NE [1 ]
机构
[1] Univ London Imperial Coll Sci & Technol, Biol Struct & Funct Sect, Div Biomed Sci, Fac Med, London SW7 2AZ, England
关键词
D O I
10.1016/j.sbi.2004.08.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protein crystallisation has gained a new strategic and commercial relevance in the post-genomic era because of its pivotal role in structural genomics. Producing high-quality crystals has always been a bottleneck to structure determination and, with the advent of proteomics, this problem is becoming increasingly acute. The task of producing suitable crystals may be tackled using two approaches. The first relies on empirical techniques that are based mainly on trial and error, and what is perceived to be the 'art' of crystallisation. The second approach is aimed at gaining an understanding of the fundamental principles that govern crystallisation; this knowledge may be applied to design experimental methodology for producing high-quality crystals of medical and industrial interest.
引用
收藏
页码:577 / 583
页数:7
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