Signal enhancement of surface plasmon resonance immunoassay using enzyme precipitation-functionalized gold nanoparticles: A femto molar level measurement of anti-glutamic acid decarboxylase antibody

被引:89
作者
Cao, Cuong [1 ]
Sim, Sang Jun [1 ]
机构
[1] Sungkyunkwan Univ, Dept Chem Engn, Suwon 440746, South Korea
关键词
enzyme precipitation; gold nanoparticles; anti-GAD antibody; surface plasmon resonance; enhancement; oligo(ethylene glycol);
D O I
10.1016/j.bios.2006.07.021
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Colloidal gold nanoparticles (AuNPs) and precipitation of an insoluble product formed by HRP-biocatalyzed oxidation of 3,3'-diaminobenzidine (DAB) in the presence of H2O2 were used to enhance the signal obtained from the surface plasmon resonance (SPR) biosensor. The AuNPs were synthesized and functionalized with HS-OEG(3)-COOH by self assembling technique. Thereafter, the HS-OEG3-COOH functionalized nanoparticles were covalently conjugated with horseradish peroxidase (HRP) and anti IgG antibody to form an enzyme-immunogold complex. Characterizations were performed by several methods: UV-vis absorption, DLS, HR-TEM and Fr-IR. The Au-anti IgG-HRP complex has been applied in enhancement of SPR immunoassay using a sensor chip constructed by 1:9 molar ratio of HS-OEG(6)-COOH and HS-OEG(3)-OH for detection of anti-GAD antibody. As a result, AuNPs showed their enhancement as being consistent with other previous studies while the enzyme precipitation using DAB substrate was applied for the first time and greatly amplified the SPR detection. The limit of detection was found as low as 0.03 ng/ml of anti-GAD antibody (or 200 fM) which is much higher than that of previous reports. This study indicates another way to enhance SPR measurement, and it is generally applicable to other SPR-based immunoassays. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1874 / 1880
页数:7
相关论文
共 37 条
[1]   Selective synthesis of heterobifunctional poly(ethylene glycol) derivatives containing both mercapto and acetal terminals [J].
Akiyama, Y ;
Otsuka, H ;
Nagasaki, Y ;
Kato, M ;
Kataoka, K .
BIOCONJUGATE CHEMISTRY, 2000, 11 (06) :947-950
[2]   Liposomes labeled with biotin and horseradish peroxidase: A probe for the enhanced amplification of antigen-antibody or oligonucleotide-DNA sensing processes by the precipitation of an insoluble product on electrodes [J].
Alfonta, L ;
Singh, AK ;
Willner, I .
ANALYTICAL CHEMISTRY, 2001, 73 (01) :91-102
[3]   Spectroscopic identification of S-Au interaction in cysteine capped gold nanoparticles [J].
Aryal, S ;
Remant, BKC ;
Dharmaraj, N ;
Bhattarai, N ;
Kim, CH ;
Kim, HY .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2006, 63 (01) :160-163
[4]   Structure and dynamics in alkanethiolate monolayers self-assembled on gold nanoparticles: A DSC, FT-IR, and deuterium NMR study [J].
Badia, A ;
Cuccia, L ;
Demers, L ;
Morin, F ;
Lennox, RB .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (11) :2682-2692
[5]   IDENTIFICATION OF THE 64K AUTOANTIGEN IN INSULIN-DEPENDENT DIABETES AS THE GABA-SYNTHESIZING ENZYME GLUTAMIC-ACID DECARBOXYLASE [J].
BAEKKESKOV, S ;
AANSTOOT, HJ ;
CHRISTGAU, S ;
REETZ, A ;
SOLIMENA, M ;
CASCALHO, M ;
FOLLI, F ;
RICHTEROLESEN, H ;
CAMILLI, PD .
NATURE, 1990, 347 (6289) :151-156
[6]   Intra- and intermonolayer hydrogen bonding in amide-functionalized alkanethiol self-assembled monolayers on gold nanoparticles [J].
Boal, AK ;
Rotello, VM .
LANGMUIR, 2000, 16 (24) :9527-9532
[7]   SYNTHESIS OF THIOL-DERIVATIZED GOLD NANOPARTICLES IN A 2-PHASE LIQUID-LIQUID SYSTEM [J].
BRUST, M ;
WALKER, M ;
BETHELL, D ;
SCHIFFRIN, DJ ;
WHYMAN, R .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1994, (07) :801-802
[8]   A strategy for sensitivity and specificity enhancements in prostate specific antigen-α1-antichymotrypsin detection based on surface plasmon resonance [J].
Cao, C ;
Kim, JP ;
Kim, BW ;
Chae, H ;
Yoon, HC ;
Yang, SS ;
Sim, SJ .
BIOSENSORS & BIOELECTRONICS, 2006, 21 (11) :2106-2113
[9]   Self-assembling of monolayer-protected gold nanoparticles [J].
Chen, SW .
JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (04) :663-667
[10]   Enhanced performance of a surface plasmon resonance immunosensor for detecting Ab-GAD antibody based on the modified self-assembled monolayers [J].
Choi, SH ;
Lee, JW ;
Sim, SJ .
BIOSENSORS & BIOELECTRONICS, 2005, 21 (02) :378-383