The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting

被引：71

作者：

Prag, Gali

Watson, Hadiya

Kim, Young C.

Beach, Bridgette M.

Ghirlando, Rodolfo

Hummer, Gerhard

Bonifacino, Juan S.

Hurley, James H. ^{[1
]}

机构：

[1] NIH, US Dept Hlth & Human Serv, Mol Biol Lab, NIDDKD, Bethesda, MD 20892 USA

[2] NIH, US Dept Hlth & Human Serv, Phys Chem Lab, NIDDKD, Bethesda, MD 20892 USA

[3] NIH, US Dept Hlth & Human Serv, Cell Biol & Metab Branch, NICHHD, Bethesda, MD 20892 USA

来源：

DEVELOPMENTAL CELL | 2007年 / 12卷 / 06期

关键词：

D O I：

10.1016/j.devcel.2007.04.013

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The yeast Vps27/Hse1 complex and the homologous mammalian Hrs/STAM complex deliver ubiquitinated transmembrane proteins to the ESCRT endosomal-sorting pathway. The Vps27/Hse1 complex directly binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes ' We have solved the crystal structure of the core responsible for the assembly of the Vps27/Hse1 complex at 3.0 angstrom resolution. The structure consists of two intertwined GAT domains, each consisting of two helices from one subunit and one from the other. The two GAT domains are connected by an antiparallel coiled coil, forming a 90 angstrom-long barbell-like structure. This structure places the domains of Vps27 and Hsel that recruit ubiquitinated cargo and deubiquitinating enzymes close to each other. Coarse-grained Monte Carlo simulations of the Vps27/Hse1 complex on a membrane show how the complex binds cooperatively to lipids and ubiquitinated membrane proteins and acts as a scaffold for ubiquitination reactions.

引用

页码：973 / 986

页数：14

共 77 条

[41] TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation [J].