Laminin inhibits A β42 fibril formation in vitro

被引：19

作者：

Monji, A ^{[1
]}

Tashiro, K

Yoshida, I

Hayashi, Y

Tashiro, N

机构：

[1] Kyushu Univ, Fac Med, Dept Neuropsychiat, Fukuoka 8128582, Japan

[2] Miyazaki Med Coll, Dept Psychiat, Miyazaki 8891601, Japan

来源：

BRAIN RESEARCH | 1998年 / 788卷 / 1-2期

关键词：

laminin; A beta 42; Alzheimer's disease; thioflavine-T fluorescence; electron microscopy;

D O I：

10.1016/S0006-8993(97)01542-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In the present study, we investigated whether or not laminin inhibits A beta 42 fibril formation in the same manner as A beta 40. Both a thioflavine-T fluorometric assay and electron microscopy by negative staining demonstrated laminin to have a concentration-dependent inhibitory effect on A beta 42 fibril formation. The amyloid fibril formation was inhibited approximately by 70% due to the presence of 1.0 mg/ml laminin co-incubated with 1.0 mg/ml A beta 42 peptide (molar ratio; A beta 42 peptide:laminin = 200:1). These results thus suggested that laminin or its derivatives may be effective as therapeutic agents to either prevent or slow down the progression of amyloidogenesis in Alzheimer's disease. (C) 1998 Elsevier Science B.V.

引用

页码：187 / 190

页数：4

共 21 条

[1] Aksenov MY, 1996, J NEUROCHEM, V66, P2050
[2] alpha 1-antichymotrypsin interaction with A beta(1-42) does not inhibit fibril formation but attenuates the peptide toxicity
Aksenov, MY
Aksenova, MV
Carney, JM
Butterfield, DA
[J]. NEUROSCIENCE LETTERS, 1996, 217 (2-3) : 117 - 120
[3] alpha-1-Antichymotrypsin interaction with A beta (1-40) inhibits fibril formation but does not affect the peptide toxicity
Aksenova, MV
Aksenov, MY
Butterfield, DA
Carney, JM
[J]. NEUROSCIENCE LETTERS, 1996, 211 (01) : 45 - 48
[4] Bronfman FC, 1996, J CELL PHYSIOL, V166, P360
[5] Laminin inhibits amyloid-beta-peptide fibrillation
Bronfman, FC
Garrido, J
Alvarez, A
Morgan, C
Inestrosa, NC
[J]. NEUROSCIENCE LETTERS, 1996, 218 (03) : 201 - 203
[6] BETA-AMYLOID FIBRILS INDUCE TAU-PHOSPHORYLATION AND LOSS OF MICROTUBULE-BINDING
BUSCIGLIO, J
LORENZO, A
YEH, J
YANKNER, BA
[J]. NEURON, 1995, 14 (04) : 879 - 888
[7] ALPHA(1)-ANTICHYMOTRYPSIN REGULATES ALZHEIMER BETA-AMYLOID PEPTIDE FIBRIL FORMATION
ERIKSSON, S
JANCIAUSKIENE, S
LANNFELT, L
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (06) : 2313 - 2317
[8] A beta 1-40 but not A beta 1-42 levels in cortex correlate with apolipoprotein E epsilon 4 allele dosage in sporadic Alzheimer's disease
Ishii, K
Tamaoka, A
Mizusawa, H
Shoji, S
Ohtake, T
Fraser, PE
Takahashi, H
Tsuji, S
Gearing, M
Mizutani, T
Yamada, S
Kato, M
StGeorgeHyslop, PH
Mirra, SS
Mori, H
[J]. BRAIN RESEARCH, 1997, 748 (1-2) : 250 - 252
[9] INHIBITION OF ALZHEIMER BETA-PEPTIDE FIBRIL FORMATION BY SERUM AMYLOID-P COMPONENT
JANCIAUSKIENE, S
DE FRUTOS, PG
CARLEMALM, E
DAHLBACK, B
ERIKSSON, S
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (44) : 26041 - 26044
[10] SEEDING ONE-DIMENSIONAL CRYSTALLIZATION OF AMYLOID - A PATHOGENIC MECHANISM IN ALZHEIMERS-DISEASE AND SCRAPIE
JARRETT, JT
LANSBURY, PT
[J]. CELL, 1993, 73 (06) : 1055 - 1058

← 1 2 3 →