Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains

被引：40

作者：

Riffard, S ^{[1
]}

Lo Presti, F ^{[1
]}

Vandenesch, F ^{[1
]}

Forey, F ^{[1
]}

Reyrolle, M ^{[1
]}

Etienne, J ^{[1
]}

机构：

[1] Fac Med RTH Laennec, UPRES EA1655, Bacteriol Lab, Ctr Natl Reference Legionella, F-69372 Lyon 08, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 01期

关键词：

D O I：

10.1128/JCM.36.1.161-167.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two methods were compared for the analysis of 48 unrelated and epidemiologically related Legionella pneumophila serogroup I isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI restriction sites and the pulsed-field gel electrophoresis (PFGE) analysis determined after DNA restriction with SfiI. Both methods demonstrated a high level: of discrimination with a similar capacity for differentiating 23 of the 24 unrelated isolates. PFGE analysis and IRS-PCR assay were both able to identify epidemiologically related isolates of L. pneumophila from three outbreaks. Hence, IRS PCR assay appears to be a reproducible (intergel reproducibility, 100%) and discriminative (discriminatory index, greater than or equal to 0.996) tool for typing of Legionella. Compared to PFGE, however, IRS-PCR presented an advantage through ease of performance and with attributes of rapidity and sensitivity of target DNA.

引用

页码：161 / 167

页数：7

共 27 条

[1] RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF RIBOSOMAL-RNA GENES FOR MOLECULAR TYPING OF MEMBERS OF THE FAMILY LEGIONELLACEAE [J].