Determination of dimethindene in human tears by high-performance liquid chromatography

A high-performance liquid chromatographic method is described for the determination of dimethindene in human tears. The rear samples were diluted in a 0.01 M hydrochloric acid-n-propanol mixture to prevent the irreversible absorption of dimethindene. The diluted samples were directly injected into the chromatographic system to avoid sample pretreatment. The validation data demonstrate that the method is specific, precise and accurate within the calibration range of 12 to 1000 ng/ml dimethindene free base. (C) 1998 Elsevier Science B.V.