The clostridial mobilisable transposons

被引:49
作者
Adams, V [1 ]
Lyras, D [1 ]
Farrow, KA [1 ]
Rood, JI [1 ]
机构
[1] Monash Univ, Dept Microbiol, Bacterial Pathogenesis Res Grp, Clayton, Vic 3800, Australia
关键词
clostridia; transposon; mobilisation; antibiotic resistance; resolvase;
D O I
10.1007/s000180200003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mobilisable transposons are transposable genetic elements that also encode mobilisation functions but are not in themselves conjugative. They rely on coresident conjugative elements to facilitate their transfer to recipient cells. Clostridial mobilisable transposons include Tn4451 and Tn4452 from Clostridium perfringens, and Tn4453 a and Tn4453 b from Clostridium difficile, all of which are closely related. and Tn5398 from C. difficile, The Tn4451 group of elements encodes resistance to chloramphenicol and is unusual in that transposition is dependent upon a large resolvase protein rather than a more conventional transposase or integrase. This group of elements also encodes the mobilisation protein TnpZ that, by acting at the RSA or oriT site located on the transposon, and in the presence of a coresident conjugative element, promotes the movement of the nonreplicating circular intermediate and of plasmids on which the transposon resides. The erythromycin resistance element Tn5398 is unique in that it encodes no readily identifiable transposition or mobilisation proteins. However, the element is still capable of intraspecific transfer between C. difficile isolates, by an unknown mechanism. The detailed analysis of these mobilisable clostridial elements provides evidence that the evolution and dissemination of antibiotic resistance genes is a complex process that may involve the interaction of genetic elements with very different properties.
引用
收藏
页码:2033 / 2043
页数:11
相关论文
共 57 条
[1]   IDENTIFICATION OF TN4451 AND TN4452, CHLORAMPHENICOL RESISTANCE TRANSPOSONS FROM CLOSTRIDIUM-PERFRINGENS [J].
ABRAHAM, LJ ;
ROOD, JI .
JOURNAL OF BACTERIOLOGY, 1987, 169 (04) :1579-1584
[2]   THE CLOSTRIDIUM-PERFRINGENS CHLORAMPHENICOL RESISTANCE TRANSPOSON TN4451 EXCISES PRECISELY IN ESCHERICHIA-COLI [J].
ABRAHAM, LJ ;
ROOD, JI .
PLASMID, 1988, 19 (02) :164-168
[3]   WORLDWIDE DISTRIBUTION OF THE CONJUGATIVE CLOSTRIDIUM-PERFRINGENS TETRACYCLINE RESISTANCE PLASMID, PCW3 [J].
ABRAHAM, LJ ;
WALES, AJ ;
ROOD, JI .
PLASMID, 1985, 14 (01) :37-46
[4]  
Alonso JC, 1996, FEMS MICROBIOL LETT, V142, P1
[5]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[6]   VIRULENCE STUDIES ON CHROMOSOMAL ALPHA-TOXIN AND THETA-TOXIN MUTANTS CONSTRUCTED BY ALLELIC EXCHANGE PROVIDE GENETIC-EVIDENCE FOR THE ESSENTIAL ROLE OF ALPHA-TOXIN IN CLOSTRIDIUM PERFRINGENS-MEDIATED GAS-GANGRENE [J].
AWAD, MM ;
BRYANT, AE ;
STEVENS, DL ;
ROOD, JI .
MOLECULAR MICROBIOLOGY, 1995, 15 (02) :191-202
[7]   MOLECULAR-GENETICS OF THE CHLORAMPHENICOL-RESISTANCE TRANSPOSON TN4451 FROM CLOSTRIDIUM-PERFRINGENS - THE TNPX SITE-SPECIFIC RECOMBINASE EXCISES A CIRCULAR TRANSPOSON MOLECULE [J].
BANNAM, TL ;
CRELLIN, PK ;
ROOD, JI .
MOLECULAR MICROBIOLOGY, 1995, 16 (03) :535-551
[8]   RELATIONSHIP BETWEEN THE CLOSTRIDIUM-PERFRINGENS CATQ GENE-PRODUCT AND CHLORAMPHENICOL ACETYLTRANSFERASES FROM OTHER BACTERIA [J].
BANNAM, TL ;
ROOD, JI .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1991, 35 (03) :471-476
[9]   THE CLOSELY-RELATED ERMB-ERMAM GENES FROM CLOSTRIDIUM-PERFRINGENS, ENTEROCOCCUS-FAECALIS (PAM-BETA-1), AND STREPTOCOCCUS-AGALACTIAE (PIP501) ARE FLANKED BY VARIANTS OF A DIRECTLY REPEATED SEQUENCE [J].
BERRYMAN, DI ;
ROOD, JI .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (08) :1830-1834
[10]   CHARACTERIZATION AND TRANSFERABILITY OF CLOSTRIDIUM-PERFRINGENS PLASMIDS [J].
BREFORT, G ;
MAGOT, M ;
IONESCO, H ;
SEBALD, M .
PLASMID, 1977, 1 (01) :52-66