Analysis of mRNA-lncRNA and mRNA-lncRNA-pathway co-expression networks based on WGCNA in developing pediatric sepsis

被引：82

作者：

Zhang, Xiaojuan ^{[1
]}

Cui, Yuqing ^{[1
]}

Ding, Xianfei ^{[1
]}

Liu, Shaohua ^{[1
]}

Han, Bing ^{[1
]}

Duan, Xiaoguang ^{[1
]}

Zhang, Haibo ^{[2
,3
]}

Sun, Tongwen ^{[1
]}

机构：

[1] Zhengzhou Univ, Henan Key Lab Crit Care Med, Zhengzhou Key Lab Sepsis,Gen ICU, Henan Engn Res Ctr Crit Care Med,Affiliated Hosp, Zhengzhou, Peoples R China

[2] Univ Toronto, Dept Anesthesia, Interdept Div Crit Care Med, Room 619,LKSKI,30 Bond St, Toronto, ON M5B 1W8, Canada

[3] Univ Toronto, Dept Physiol, Interdept Div Crit Care Med, Room 619,LKSKI,30 Bond St, Toronto, ON M5B 1W8, Canada

来源：

BIOENGINEERED | 2021年 / 12卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Pediatric sepsis; mRNA; lncRNA; WGCNA; diagnostic markers; COMPLEMENT RECEPTOR 1; SEPTIC SHOCK; SIGNALING PATHWAY; NONCODING RNA; LUNG INJURY; ACTIVATION; EXPRESSION; MORTALITY; 5-LIPOXYGENASE; CD11B;

D O I：

10.1080/21655979.2021.1908029

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Pediatric sepsis is a great threat to death worldwide. However, the pathogenesis has not been clearly understood until now in sepsis. This study identified differentially expressed mRNAs and lncRNAs based on Gene Expression Omnibus (GEO) database. And the weighted gene co-expression network analysis (WGCNA) was performed to explore co-expression modules associated with pediatric sepsis. Then, Gene Ontology (GO), KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway, mRNA-lncRNA and mRNA-lncRNA-pathway co-expression network analysis was conducted in selected significant module. A total of 1941 mRNAs and 225 lncRNAs were used to conduct WGCNA. And turquoise module was selected as a significant module that was associated with particular traits. The mRNAs functions associated with many vital processes were also shown by GO and KEGG pathway analysis in the turquoise module. Finally, 15 mRNAs (MAPK14, ITGAM, HK3, ALOX5, CR1, HCK, NCF4, PYGL, FLOT1, CARD6, NLRC4, SH3GLB1, PGS1, RAB31, LTB4R) and 4 lncRNAs (GSEC, NONHSAT160878.1, XR_926068.1 and RARA-AS1) were selected as hub genes in mRNA-lncRNA-Pathway co-expression network. We identified 15 mRNAs and 4 lncRNAs as diagnostic markers, which have potential functions in pediatric sepsis. Our study provides more directions to study the molecular mechanism of pediatric sepsis.

引用

页码：1457 / 1470

页数：14

共 39 条

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