A sensitive immunoassay based on electropolymerized films by capacitance measurements for direct detection of immunospecies

被引：45

作者：

Wu, ZS ^{[1
]}

Li, JS ^{[1
]}

Deng, T ^{[1
]}

Luo, MH ^{[1
]}

Shen, GL ^{[1
]}

Yu, RQ ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2005年 / 337卷 / 02期

基金：

中国国家自然科学基金;

关键词：

capacitive immunosensor; human serum albumin; polytyramine; alternating current impedance;

D O I：

10.1016/j.ab.2004.10.051

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fabrication of a capacitive immunosensor based on electropolymerized polytyramine (Pty) film for the direct detection of human serum albumin (HSA) without any labeling is described. The capacitance change of the heterostructures, Pty films/covalently bonded antibodies/buffered medium, is utilized for monitoring the specific antibody-antigen interaction. The Pty films are ultrathin and the HSA assay is nearly specific. Experimental parameters affecting antibody immobilization and the sensing of HSA are investigated in detail and optimized. This capacitive sensor prepared with the present method can provide high sensitivity. Under the optimized experimental conditions, a linear calibration curve in the concentration range 1.84-368.6 ng/ml when plotted vs the logarithm of the antigen concentration is obtained and the detection limit (S/N = 3) is 1.60 ng/ml. After an acidic washing the present system can be used again. The applicability

引用

页码：308 / 315

页数：8

共 29 条

[1] DIRECT DETECTION OF IMMUNOSPECIES BY CAPACITANCE MEASUREMENTS [J].