Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection

被引：99

作者：

Bontidean, I

Ahlqvist, J

Mulchandani, A

Chen, W

Bae, W

Mehra, RK

Mortari, A

Csöregi, E

机构：

[1] Lund Univ, Ctr Chem & Chem Engn, Dept Biotechnol, SE-22100 Lund, Sweden

[2] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2003年 / 18卷 / 5-6期

关键词：

biosensors; heavy metals; phytochelatins; capacitance;

D O I：

10.1016/S0956-5663(03)00026-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)(20)Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Ph2+, Cu2+ and Zn2+ ions in concentration range of 100 fM- 10 mM, and the order of sensitivity was S-Zn > S-Cu > S-Hg much greater than S-Cd congruent to S-Pb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：547 / 553

页数：7

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