Gold glyconanoparticles:: Synthetic polyvalent ligands mimicking glycocalyx-like surfaces as tools for glycobiological studies

被引:227
作者
Barrientos, AG
de la Fuente, JM
Rojas, TC
Fernández, A
Penadés, S
机构
[1] CSIC, Inst Invest Quim, Grp Carbohidratos, Seville 41092, Spain
[2] CSIC, Inst Ciencias Mat, Seville 41092, Spain
关键词
carbohydrates; gold; multivalency; nanoparticles; nanotechnology;
D O I
10.1002/chem.200204544
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A simple and versatile methodology is described for tailoring sugar-functionalised gold nanoclusters (glyconanoparticles) that have 3D polyvalent carbohydrate display and globular shapes. This methodology allows the preparation of glyconanoparticles with, biologically significant oligosaccharides as well as with differing carbohydrate density. Fluorescent glyconanoparticles have been also prepared for labelling cells in biological tests. The materials are water soluble, stable under physiological conditions and present an exceptional small core size. All of them have been characterised by H-1 NMR, UV and IR spectroscopy, TEM and elemental analysis. Their highly polyvalent network can mimic glycosphingolipid clustering and interactions at the plasma membrane, providing an controlled system for glycobiological studies. Furthermore, they are useful building blocks for the design of nanomaterials.
引用
收藏
页码:1909 / 1921
页数:13
相关论文
共 45 条
  • [31] 2-W
  • [32] Rojo J, 2002, TOP CURR CHEM, V218, P45
  • [33] ROJO J, UNPUB
  • [34] ANOMERIC-OXYGEN ACTIVATION FOR GLYCOSIDE SYNTHESIS - THE TRICHLOROACETIMIDATE METHOD
    SCHMIDT, RR
    KINZY, W
    [J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, VOL 50, 1994, 50 : 21 - 123
  • [35] Adhesion-related glycocalyx study: quantitative approach with imaging-spectrum in the energy filtering transmission electron microscope (EFTEM)
    Soler, M
    Desplat-Jego, S
    Vacher, B
    Ponsonnet, L
    Fraterno, M
    Bongrand, P
    Martin, JM
    Foa, C
    [J]. FEBS LETTERS, 1998, 429 (01) : 89 - 94
  • [36] Programmed materials synthesis with DNA
    Storhoff, JJ
    Mirkin, CA
    [J]. CHEMICAL REVIEWS, 1999, 99 (07) : 1849 - 1862
  • [37] SACCHARIDE ORIENTATION AT THE CELL-SURFACE AFFECTS GLYCOLIPID RECEPTOR FUNCTION
    STROMBERG, N
    NYHOLM, PG
    PASCHER, I
    NORMARK, S
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) : 9340 - 9344
  • [38] Water-soluble, isolable gold clusters protected by tiopronin and coenzyme A monolayers
    Templeton, AC
    Chen, SW
    Gross, SM
    Murray, RW
    [J]. LANGMUIR, 1999, 15 (01) : 66 - 76
  • [39] Tromas C, 2001, ANGEW CHEM INT EDIT, V40, P3052, DOI 10.1002/1521-3773(20010817)40:16<3052::AID-ANIE3052>3.0.CO
  • [40] 2-Q