Development of a specific monoclonal antibody for grouper (Epinephelus guaza) identification by an indirect enzyme-linked immunosorbent assay

被引：11

作者：

Asensio, L ^{[1
]}

González, I ^{[1
]}

Rodríguez, MA ^{[1
]}

Mayoral, B ^{[1
]}

López-Calleja, I ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol Higiene & Tecnol Alimentos 3, E-28040 Madrid, Spain

来源：

JOURNAL OF FOOD PROTECTION | 2003年 / 66卷 / 05期

关键词：

D O I：

10.4315/0362-028X-66.5.886

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Identification of fish species adulteration is important for consumer protection and the enforcement of food-labeling laws. A monoclonal antibody (MAb) generated against soluble muscle proteins from grouper (Epinephelus guaza) has been used in two indirect enzyme-linked immunosorbent assay (ELISA) formats (microtiter plates and immunostick tubes) for the rapid authentication of grouper fillets. The 3D12 MAb was produced with the use of the hybridoma technique and tested against several commonly consumed fish species by ELISA. The 3D12 MAb specifically reacted with grouper samples and could be useful for the discrimination of grouper among other, less-valued, fish species sold in the marketplace.

引用

页码：886 / 889

页数：4

共 19 条

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