Versatile insertion plasmids for targeted genome manipulations in bacteria: Isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome

被引：167

作者：

Posfai, G

Koob, MD

Kirkpatrick, HA

Blattner, FR

机构：

[1] UNIV MINNESOTA, DEPT NEUROL, MINNEAPOLIS, MN 55455 USA

[2] UNIV WISCONSIN, DEPT GENET, MADISON, WI 53706 USA

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 13期

关键词：

D O I：

10.1128/jb.179.13.4426-4428.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was as excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.

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页码：4426 / 4428

页数：3

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