Developmental fate of embryonic germ cells (EGCs), in vivo and in vitro

被引:29
作者
Durcova-Hills, G [1 ]
Wianny, F [1 ]
Merriman, J [1 ]
Zernicka-Goetz, M [1 ]
McLaren, A [1 ]
机构
[1] Wellcome Trust Canc Res UK Inst Canc & Dev Biol, Cambridge CB2 1QR, England
基金
英国惠康基金;
关键词
primordial germ cells; embryonic germ cells; chimera; green fluorescence protein; aggregates; differentiation;
D O I
10.1046/j.1432-0436.2003.710204.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Embryonic germ cells (EGCs) derived from mouse primordial germ cells (PGCs) are known both to colonize all cell lineages of the fetus and to make tumors in vivo. When aggregated with eight-cell embryos, EGCs from a new EGC line expressing green fluorescent protein (GFP) were found to contribute preferentially to the epiblast but unexpectedly were also capable of colonizing primary endoderm. When injected under the kidney capsule, EGCs derived from 12.5 days post coitum (dpc) PGCs formed differentiated tumors. The ability of EGCs to differentiate in an organ culture system depends upon their partners in cell culture. When EGCs, marked with a LacZ transgene, were mixed with disaggregated and reaggregated mouse fetal lung in an organ culture system, they remained undifferentiated. In urogenital ridge reaggregates on the other hand, some EGCs were capable of differentiating to form small epithelial cysts.
引用
收藏
页码:135 / 141
页数:7
相关论文
共 29 条
[21]   PURIFICATION OF MOUSE PRIMORDIAL GERM-CELLS BY MINIMACS MAGNETIC SEPARATION SYSTEM [J].
PESCE, M ;
DEFELICI, M .
DEVELOPMENTAL BIOLOGY, 1995, 170 (02) :722-725
[22]   LONG-TERM PROLIFERATION OF MOUSE PRIMORDIAL GERM-CELLS IN CULTURE [J].
RESNICK, JL ;
BIXLER, LS ;
CHENG, LZ ;
DONOVAN, PJ .
NATURE, 1992, 359 (6395) :550-551
[23]   Primordial germ cell-derived mouse embryonic germ (EG) cells in vitro resemble undifferentiated stem cells with respect to differentiation capacity and cell cycle distribution [J].
Rohwedel, J ;
Sehlmeyer, U ;
Shan, J ;
Meister, A ;
Wobus, AM .
CELL BIOLOGY INTERNATIONAL, 1996, 20 (08) :579-587
[24]   Derivation of pluripotent stem cells horn cultured human primordial germ cells [J].
Shamblott, MJ ;
Axelman, J ;
Wang, SP ;
Bugg, EM ;
Littlefield, JW ;
Donovan, PJ ;
Blumenthal, PD ;
Huggins, GR ;
Gearhart, JD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13726-13731
[25]   Isolation of pluripotent stem cells from cultured porcine primordial germ cells [J].
Shim, H ;
GutierrezAdan, A ;
Chen, LR ;
BonDurant, RH ;
Behboodi, E ;
Anderson, GB .
BIOLOGY OF REPRODUCTION, 1997, 57 (05) :1089-1095
[26]   STEM-CELLS FROM PRIMORDIAL GERM-CELLS CAN REENTER THE GERM-LINE [J].
STEWART, CL ;
GADI, I ;
BHATT, H .
DEVELOPMENTAL BIOLOGY, 1994, 161 (02) :626-628
[27]   A MOUSE EMBRYONIC STEM-CELL LINE SHOWING PLURIPOTENCY OF DIFFERENTIATION IN EARLY EMBRYOS AND UBIQUITOUS BETA-GALACTOSIDASE EXPRESSION [J].
SUEMORI, H ;
KADODAWA, Y ;
GOTO, K ;
ARAKI, I ;
KONDOH, H ;
NAKATSUJI, N .
CELL DIFFERENTIATION AND DEVELOPMENT, 1990, 29 (03) :181-186
[28]   Epigenotype switching of imprintable loci in embryonic germ cells [J].
Tada, T ;
Tada, M ;
Hilton, K ;
Barton, SC ;
Sado, T ;
Takagi, N ;
Surani, MA .
DEVELOPMENT GENES AND EVOLUTION, 1998, 207 (08) :551-561
[29]  
ZernickaGoetz M, 1997, DEVELOPMENT, V124, P1133